FastQCFastQC Report
Wed 25 May 2016
SRR1780022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59302
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2103335.46760648881994Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG37886.387642912549324No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC19473.2831944959697816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14882.5091902465346867No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT12042.030285656470271Illumina PCR Primer Index 1 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC7321.234359718053354No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT5040.8498870189875551Illumina PCR Primer Index 1 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4850.8178476273987387Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT3720.6272975616336717TruSeq Adapter, Index 1 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC3490.5885130349735254Illumina PCR Primer Index 1 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC2080.3507470237091498Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT2030.3423156048699875No Hit
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT1940.32713905095949547Illumina PCR Primer Index 1 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC1840.31027621328117094No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCGT1720.2900408080671815No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT1460.2461974301035378No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG1380.23270715996087823No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAGT1290.21753060605038615No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC1280.2158443222825537Illumina PCR Primer Index 1 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG1200.20235405213989408No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT930.15682439040841792No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCCAACACGATCTCGTATGCCG880.14839297156925568No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGA870.14670668780142324No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGAACTCGTATGCCGT830.13996155273009342No Hit
GCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG810.13658898519442852No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG800.13490270142659605No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCG800.13490270142659605No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGAATGCCGT750.12647128258743381No Hit
CTTATACACATCTCCGAGACCACGAGACCCAACACGATCTCGTATGCCGT680.11466729621260666TruSeq Adapter, Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCG680.11466729621260666No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAG680.11466729621260666No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT660.11129472867694176Illumina PCR Primer Index 1 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT650.10960844490910931Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGCCCCAACACGATCTCGTATGCCGT650.10960844490910931Illumina PCR Primer Index 1 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGCCCCAACACGATCTCGTATGCC650.10960844490910931No Hit
TCTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT620.10454959360561196Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATC252.7968235E-670.0464233
AAAACGA207.841033E-570.0464231
GATATGC150.002208662770.0464122
CTTTGCA150.002208662770.0464129
TCCGAGA550.069.9873613
ACTGCTT301.0088843E-769.9873553
GCCTAAT150.00221607369.9873570
CCGTCTA252.8108989E-669.9873547
AAGGGCG150.00221607369.987358
AGTATGC150.00221607369.9873541
TTCCTAT150.00221607369.987355
TCAAAAA150.00221607369.9873559
CCTTATA301.0088843E-769.987351
AATGCCG301.0088843E-769.9873543
TCTTTAT900.069.987351
GACCACG401.3096724E-1069.9873518
CTACTGC301.0088843E-769.9873551
CGTCTAC252.8108989E-669.9873548
TCTTAAT401.3096724E-1069.987351
CTTTATA950.066.303812
Produced by FastQC (version 0.11.5)