##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780022_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59302 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.5263734781289 32.0 27.0 32.0 22.0 32.0 2 30.523422481535192 32.0 32.0 32.0 27.0 32.0 3 30.885012309871506 32.0 32.0 32.0 27.0 32.0 4 30.667903274763077 32.0 32.0 32.0 27.0 32.0 5 30.867694175575867 32.0 32.0 32.0 27.0 32.0 6 33.48242892313919 37.0 32.0 37.0 22.0 37.0 7 35.025193079491416 37.0 37.0 37.0 32.0 37.0 8 33.01129810124448 37.0 32.0 37.0 22.0 37.0 9 34.93335806549526 37.0 37.0 37.0 32.0 37.0 10-11 34.92167211898418 37.0 37.0 37.0 29.5 37.0 12-13 35.21112272773263 37.0 37.0 37.0 32.0 37.0 14-15 35.012815756635526 37.0 37.0 37.0 29.5 37.0 16-17 33.4198677953526 37.0 34.5 37.0 24.5 37.0 18-19 33.79470338268524 37.0 34.5 37.0 24.5 37.0 20-21 34.96400627297562 37.0 37.0 37.0 29.5 37.0 22-23 34.32575629826987 37.0 37.0 37.0 27.0 37.0 24-25 34.12735658156555 37.0 37.0 37.0 27.0 37.0 26-27 33.87772756399447 37.0 34.5 37.0 24.5 37.0 28-29 33.62135341135206 37.0 34.5 37.0 24.5 37.0 30-31 33.68341708542714 37.0 34.5 37.0 24.5 37.0 32-33 32.852003305116185 37.0 32.0 37.0 20.0 37.0 34-35 33.86676671950356 37.0 34.5 37.0 24.5 37.0 36-37 33.3566574483154 37.0 32.0 37.0 22.0 37.0 38-39 32.75035411959125 37.0 32.0 37.0 22.0 37.0 40-41 33.933130417186604 37.0 37.0 37.0 24.5 37.0 42-43 33.831573977268896 37.0 37.0 37.0 24.5 37.0 44-45 34.0341894033928 37.0 34.5 37.0 27.0 37.0 46-47 33.45640956460153 37.0 32.0 37.0 22.0 37.0 48-49 32.34030049576743 37.0 29.5 37.0 17.5 37.0 50-51 33.13047620653603 37.0 32.0 37.0 22.0 37.0 52-53 32.73305284813328 37.0 32.0 37.0 22.0 37.0 54-55 33.136192708508986 37.0 32.0 37.0 22.0 37.0 56-57 32.04588378132272 37.0 32.0 37.0 17.5 37.0 58-59 32.33653165154632 37.0 32.0 37.0 17.5 37.0 60-61 31.872432632963474 37.0 29.5 37.0 17.5 37.0 62-63 33.327366699268154 37.0 32.0 37.0 24.5 37.0 64-65 33.71819668813868 37.0 32.0 37.0 27.0 37.0 66-67 33.67424370173013 37.0 32.0 37.0 27.0 37.0 68-69 33.19597989949749 37.0 32.0 37.0 22.0 37.0 70-71 29.873090283632926 34.5 22.5 37.0 13.0 37.0 72-73 29.65546693197531 37.0 24.5 37.0 13.0 37.0 74-75 30.935862196890493 37.0 27.0 37.0 13.0 37.0 76 31.318994300360863 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.2215605170307704 11101 2 -0.28693479742511485 11101 3 -1.1452567948292902 11101 4 -1.3010637519065469 11101 5 -1.1003221483115908 11101 6 -1.5215391612969036 11101 7 -1.4713254624791077 11101 8 -0.3767688158553284 11101 9 0.739965628634458 11101 10-11 -1.2450276024570286 11101 12-13 -0.19328882081610033 11101 14-15 -0.16195311028569392 11101 16-17 0.6266392681463202 11101 18-19 -1.532536286398006 11101 20-21 -0.6884137971639959 11101 22-23 -2.8488195642107357 11101 24-25 -1.3706909635011044 11101 26-27 -1.182753394407662 11101 28-29 0.11540568419231789 11101 30-31 1.8547788475061324 11101 32-33 0.9008110937101605 11101 34-35 -1.36895947262569 11101 36-37 -0.4155787775449795 11101 38-39 -0.6397625683708554 11101 40-41 -2.24763929462096 11101 42-43 -1.176680959061656 11101 44-45 0.32531656116103846 11101 46-47 -0.43020454897612836 11101 48-49 -1.4970997414816125 11101 50-51 -1.1843938828801441 11101 52-53 -1.7269366128166261 11101 54-55 -0.8412752521237294 11101 56-57 0.7396434693109342 11101 58-59 -0.3302816000021238 11101 60-61 -3.167786060177324 11101 62-63 0.2167432667140048 11101 64-65 0.1333893635699397 11101 66-67 -0.8392712149396573 11101 68-69 -1.4553250587339974 11101 70-71 -1.1809067686703614 11101 72-73 -3.684975513167945 11101 74-75 -1.3313145920743956 11101 76 0.8104606915855896 11102 1 -0.5129222415899193 11102 2 -0.1708428434021272 11102 3 0.22600757298680207 11102 4 -0.878075246159419 11102 5 -1.833655481644925 11102 6 1.1658171605421757 11102 7 0.2666055720036553 11102 8 -0.6675734135564824 11102 9 0.2112299964505553 11102 10-11 -0.1846827748708222 11102 12-13 -0.263978475988516 11102 14-15 0.020230797760284247 11102 16-17 0.3720415669968915 11102 18-19 0.716314288314635 11102 20-21 0.2851494212268122 11102 22-23 1.2189965277432862 11102 24-25 -0.2752886646505317 11102 26-27 -0.07010971624674056 11102 28-29 0.707359707180828 11102 30-31 -2.5388993134134026 11102 32-33 -0.606085458013979 11102 34-35 -0.15861464503948497 11102 36-37 0.016030417857322732 11102 38-39 2.0200075465716765 11102 40-41 1.240291739861803 11102 42-43 0.9141236386394951 11102 44-45 -1.905717921597578 11102 46-47 -1.8635378823094584 11102 48-49 -1.7292836495275878 11102 50-51 -0.516003078282445 11102 52-53 -1.0740630495982373 11102 54-55 -2.52403387281338 11102 56-57 -2.5448392893097562 11102 58-59 -2.0055689563239625 11102 60-61 -1.2815791636256044 11102 62-63 0.31731797935768924 11102 64-65 -0.7206336249358074 11102 66-67 -1.554213743675291 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11104 26-27 -0.17355799210881173 11104 28-29 -1.4478127066122823 11104 30-31 -1.7802786237582282 11104 32-33 -1.7957406304277654 11104 34-35 1.1517301825467214 11104 36-37 0.7056855902711163 11104 38-39 -0.8937855568766011 11104 40-41 -0.018328949793371407 11104 42-43 -2.2755315337742985 11104 44-45 -0.8195110250458555 11104 46-47 -0.4152620202404904 11104 48-49 -0.7292836495275878 11104 50-51 0.7253762320623878 11104 52-53 -2.1602699461499597 11104 54-55 -0.6792062866064867 11104 56-57 -1.5103565306890658 11104 58-59 -1.1262586114963753 11104 60-61 0.16669669844336354 11104 62-63 -0.5102682275388659 11104 64-65 0.5207456854090182 11104 66-67 1.2561310839109154 11104 68-69 0.4808818378177335 11104 70-71 -1.5699872284404712 11104 72-73 -1.363136432708174 11104 74-75 -1.6761421782812924 11104 76 -0.983792181977627 11105 1 -0.06667477708687741 11105 2 -0.2418367582094305 11105 3 -0.7354528732606624 11105 4 -0.5677304185732126 11105 5 0.31340334188448793 11105 6 -1.1607548475714111 11105 7 0.8345568904620677 11105 8 1.4683292233603567 11105 9 0.8968283737324967 11105 10-11 -0.06855701422173865 11105 12-13 0.10671117918389683 11105 14-15 0.5988312034398007 11105 16-17 0.03938436618553709 11105 18-19 0.5890323410529703 11105 20-21 0.18119404597325683 11105 22-23 0.09627847500494369 11105 24-25 -0.03441645369717605 11105 26-27 -0.6847141787213857 11105 28-29 0.5075625469374216 11105 30-31 -0.9197309564154281 11105 32-33 0.40963462312193144 11105 34-35 -1.2189594726256914 11105 36-37 0.14128396755306483 11105 38-39 -0.2583900193512463 11105 40-41 -0.35352164756213256 11105 42-43 -0.10413193945381494 11105 44-45 -0.6364481447213066 11105 46-47 -1.4517731764271034 11105 48-49 -0.3078840552071007 11105 50-51 0.10874337202181295 11105 52-53 -0.1602699461499597 11105 54-55 -1.2912752521237252 11105 56-57 0.21023170460505014 11105 58-59 0.7530517333312119 11105 60-61 0.16162570452855718 11105 62-63 -2.2146292823056015 11105 64-65 0.3853501478836634 11105 66-67 -0.19417317572396797 11105 68-69 -1.120030941086938 11105 70-71 -0.1622793176899684 11105 72-73 2.2463970358516647 11105 74-75 -0.3313145920743956 11105 76 0.8143822602130406 11106 1 -0.6889657987587015 11106 2 -0.9693909377759908 11106 3 -0.8066603036012197 11106 4 1.0375327393215237 11106 5 0.14002872888138995 11106 6 1.0977590843171328 11106 7 1.2076219059419486 11106 8 -0.1802775877851559 11106 9 0.6522463303888486 11106 10-11 0.6365513449113962 11106 12-13 -0.27486776818452796 11106 14-15 -0.9716022330927103 11106 16-17 0.023130496216495544 11106 18-19 -0.3544661109594145 11106 20-21 0.025621290555299936 11106 22-23 -0.5918020203510892 11106 24-25 -0.3347260512204002 11106 26-27 -0.817841113705903 11106 28-29 -0.6038925614217163 11106 30-31 -0.08381764372193601 11106 32-33 -1.3465573273424667 11106 34-35 -0.17948578841517104 11106 36-37 1.2782808715778273 11106 38-39 -0.5757274806515582 11106 40-41 -1.3581656104104312 11106 42-43 -0.5336985029213039 11106 44-45 0.2226849822136714 11106 46-47 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40-41 0.1245829276012671 21611 42-43 0.5233190409383397 21611 44-45 1.3697610056054899 21611 46-47 0.9697954510238702 21611 48-49 0.9806780362961618 21611 50-51 0.21005056156429447 21611 52-53 1.2619522760722646 21611 54-55 0.7920580812096105 21611 56-57 0.3563101359776013 21611 58-59 1.3974961777756576 21611 60-61 0.7322139398226746 21611 62-63 0.9000766000473419 21611 64-65 1.2111671413477225 21611 66-67 0.38295100728256415 21611 68-69 1.4835638301548926 21611 70-71 2.135759897996305 21611 72-73 0.5705800423876113 21611 74-75 -0.19798125874106276 21611 76 -0.5784281973032961 21612 1 1.2044873462990644 21612 2 -0.19018682994543923 21612 3 -0.2046063883252245 21612 4 0.4810500692316637 21612 5 0.21512500615995123 21612 6 1.984151895613664 21612 7 1.6408696594721164 21612 8 2.0077840296731253 21612 9 1.0976892058702319 21612 10-11 0.6269236170551693 21612 12-13 0.4176867889399958 21612 14-15 0.48154282467365306 21612 16-17 0.4002165039186778 21612 18-19 -0.712617587211021 21612 20-21 -0.01930810610708278 21612 22-23 0.17231864717137313 21612 24-25 -0.38126006919216593 21612 26-27 -0.41283469522067406 21612 28-29 1.4121536516719928 21612 30-31 -0.22855448582719617 21612 32-33 -0.1430913453142324 21612 34-35 0.45786979566698705 21612 36-37 2.3482423606664042 21612 38-39 -0.7974861456066265 21612 40-41 -0.7269075873038844 21612 42-43 0.21437595150744926 21612 44-45 0.4521458294537197 21612 46-47 0.44134016647102925 21612 48-49 -0.727181042294621 21612 50-51 0.8956874179328622 21612 52-53 1.0470471270207682 21612 54-55 0.6538466990957801 21612 56-57 -0.3762101892256524 21612 58-59 0.44817368455072426 21612 60-61 0.12001881787145052 21612 62-63 1.0927595268766126 21612 64-65 -0.5511634819585183 21612 66-67 -0.2278891011185209 21612 68-69 -0.28825188800229284 21612 70-71 0.2609631500288252 21612 72-73 0.3475448120353093 21612 74-75 0.19307565182804254 21612 76 2.014525732235999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 1.0 17 3.0 18 15.0 19 18.0 20 48.0 21 84.0 22 152.0 23 262.0 24 451.0 25 645.0 26 863.0 27 1283.0 28 1618.0 29 2225.0 30 2986.0 31 4287.0 32 6288.0 33 9581.0 34 13985.0 35 12456.0 36 2050.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.51572486130082 11.230839277583852 14.856410515842903 56.397025345272425 2 10.208280630744582 8.644911038030187 62.22278438316891 18.92402394805633 3 10.438096522882871 13.007992985059527 64.03999865097299 12.513911841084619 4 7.819297831439075 51.831304171866044 27.28744393106472 13.061954065630163 5 7.80243499376075 25.44264948905602 59.851269771677174 6.903645745506054 6 12.593804280004722 62.25231454156056 15.612404930943185 9.541476247491525 7 12.092340899126505 22.99585174193113 12.96246332332805 51.94934403561431 8 10.778725843985026 63.36042629253651 8.760244173889582 17.100603689588883 9 10.478567333310849 17.554214023135813 7.104313513878116 64.86290512967521 10-11 11.309062088968332 38.47172102121345 32.49553134801524 17.723685541802976 12-13 12.533304104414691 10.659842838352839 38.99446898924151 37.81238406799096 14-15 13.319955482108528 9.459208795656133 14.471687295538093 62.749148426697246 16-17 38.58807460119389 31.875822063336816 7.8504940811439745 21.685609254325318 18-19 40.84129406507424 17.82669915600786 7.968601131505948 33.36340564741195 20-21 15.340966577855722 9.59579778085056 7.806650703180332 67.25658493811338 22-23 13.185275121836057 37.00190553279034 7.757879293772449 42.05494005160115 24-25 38.59813320517036 36.01463756018179 8.035480906247102 17.351748328400745 26-27 40.950413223140494 39.50413223140496 7.481868780570079 12.063585764884465 28-29 13.74096654776662 19.478526314014182 8.07930042922074 58.701206708998455 30-31 10.412169454937938 34.03939557474366 8.04860361575823 47.49983135456017 32-33 11.938511354149977 43.34561644644197 7.822684689394642 36.89318751001341 34-35 11.39433698163482 40.69767441860465 8.998768909051048 38.90921969070948 36-37 37.0662035007251 34.38922801929108 10.484469326498264 18.06009915348555 38-39 14.900846514451452 16.292030622913224 36.290512967522176 32.51660989511315 40-41 11.167508410411203 9.022537372579107 40.681938905423976 39.12801531158571 42-43 38.29414095764862 8.856437020985979 36.499919900845676 16.34950212051972 44-45 13.602408013220465 34.99460389194294 39.96914100704866 11.433847087787933 46-47 37.82450696862642 11.476944090790283 18.66647555289495 32.032073387688335 48-49 35.47182219823952 8.239182489629354 11.779535260193585 44.50946005193754 50-51 16.76250379413848 8.346261508886716 38.62180027655054 36.26943442042427 52-53 9.766112441401638 9.376580891032344 63.6825064921925 17.174800175373512 54-55 9.357425316818576 9.079181457154661 44.39422939098321 37.16916383504355 56-57 36.644632558766986 8.31843782671748 19.08957539374726 35.94735422076827 58-59 12.606976332408665 12.510855726342948 57.86629117798332 17.015876763265066 60-61 35.6733331084955 35.55782267039898 19.63002934133756 9.138814879767967 62-63 11.827694071819431 70.26129187288686 8.188663018642025 9.722351036651688 64-65 8.431774298265585 74.25610671253551 7.150144604929215 10.161974384269682 66-67 8.950252951096122 74.1273187183811 7.014333895446881 9.908094435075885 68-69 11.825908063808978 71.48831405348892 6.8825671984081485 9.803210684293953 70-71 31.66840915989343 51.028633098377796 6.463525682101784 10.839432059626994 72-73 69.95860138445065 12.82513933037107 6.3050681685960726 10.911191116582211 74-75 74.0375048483111 9.266598087657464 6.266547496585102 10.429349567446332 76 74.21797271546853 9.080791217686041 6.214060471155629 10.487175595689786 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.0 8 0.0 9 0.5 10 0.5 11 1.0 12 2.0 13 1.0 14 0.0 15 0.5 16 2.5 17 4.0 18 3.0 19 14.5 20 26.5 21 26.0 22 18.5 23 36.5 24 68.5 25 75.0 26 84.5 27 99.0 28 123.5 29 143.0 30 150.0 31 169.0 32 234.0 33 287.0 34 326.5 35 416.0 36 450.0 37 434.0 38 488.0 39 609.5 40 700.0 41 795.0 42 867.0 43 997.5 44 1189.0 45 1431.0 46 1612.0 47 2731.0 48 8059.0 49 16506.5 50 20745.0 51 11706.5 52 2383.0 53 1455.5 54 813.0 55 815.0 56 779.5 57 704.5 58 667.0 59 642.5 60 568.5 61 523.0 62 527.0 63 475.0 64 382.0 65 317.5 66 273.0 67 252.0 68 223.0 69 156.0 70 109.5 71 101.0 72 107.0 73 104.5 74 77.0 75 58.0 76 49.5 77 34.0 78 26.0 79 25.0 80 18.0 81 14.5 82 12.5 83 7.0 84 6.5 85 5.0 86 4.0 87 4.0 88 3.0 89 3.0 90 3.5 91 4.0 92 5.0 93 8.0 94 11.0 95 22.5 96 34.0 97 41.0 98 104.5 99 737.5 100 1314.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0016862837678324508 2 0.011803986374827156 3 0.0 4 0.0 5 0.0 6 0.005058851303497353 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.431418839162254E-4 20-21 0.0 22-23 0.0016862837678324508 24-25 0.0042157094195811266 26-27 0.02023540521398941 28-29 0.014333412026575832 30-31 0.010117702606994705 32-33 0.01096084449091093 34-35 0.008431418839162253 36-37 0.0 38-39 0.0 40-41 8.431418839162254E-4 42-43 8.431418839162254E-4 44-45 0.0 46-47 8.431418839162254E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0025294256517486764 56-57 0.0 58-59 0.0025294256517486764 60-61 0.0 62-63 8.431418839162254E-4 64-65 0.0042157094195811266 66-67 0.0033725675356649017 68-69 0.0 70-71 0.0 72-73 8.431418839162254E-4 74-75 0.005058851303497353 76 0.0016862837678324508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.255674344878756 #Duplication Level Percentage of deduplicated Percentage of total 1 70.1462474822664 13.507132980337932 2 8.23189421140205 3.1702134835250075 3 4.632629827480515 2.6761323395500995 4 3.8970137490148002 3.0015851067417625 5 3.3628163587004116 3.237664834238305 6 2.7235309571766355 3.1466055107753537 7 2.276906909536737 3.0690364574550606 8 1.4362028198616341 2.2124043033961756 9 1.1384534547683685 1.9729520083639676 >10 1.8215255276293898 5.2898721796903985 >50 0.1576320168140818 2.1854237631108564 >100 0.11384534547683686 4.768810495430171 >500 0.017514668534897977 2.084246737040909 >1k 0.035029337069795954 14.210313311524065 >5k 0.0 0.0 >10k+ 0.008757334267448989 35.46760648881994 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT 21033 35.46760648881994 Illumina PCR Primer Index 1 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG 3788 6.387642912549324 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC 1947 3.2831944959697816 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1488 2.5091902465346867 No Hit ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT 1204 2.030285656470271 Illumina PCR Primer Index 1 (95% over 24bp) TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC 732 1.234359718053354 No Hit CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT 504 0.8498870189875551 Illumina PCR Primer Index 1 (95% over 21bp) ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT 485 0.8178476273987387 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT 372 0.6272975616336717 TruSeq Adapter, Index 1 (95% over 23bp) CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC 349 0.5885130349735254 Illumina PCR Primer Index 1 (95% over 22bp) TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC 208 0.3507470237091498 Illumina PCR Primer Index 1 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT 203 0.3423156048699875 No Hit TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT 194 0.32713905095949547 Illumina PCR Primer Index 1 (95% over 23bp) TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC 184 0.31027621328117094 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCGT 172 0.2900408080671815 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT 146 0.2461974301035378 No Hit CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG 138 0.23270715996087823 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAGT 129 0.21753060605038615 No Hit CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC 128 0.2158443222825537 Illumina PCR Primer Index 1 (95% over 22bp) CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG 120 0.20235405213989408 No Hit CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT 93 0.15682439040841792 No Hit TCTTATACACATCTCCGAGCCCACGAGCCCCAACACGATCTCGTATGCCG 88 0.14839297156925568 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGA 87 0.14670668780142324 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAACACGAACTCGTATGCCGT 83 0.13996155273009342 No Hit GCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG 81 0.13658898519442852 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG 80 0.13490270142659605 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCG 80 0.13490270142659605 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGAATGCCGT 75 0.12647128258743381 No Hit CTTATACACATCTCCGAGACCACGAGACCCAACACGATCTCGTATGCCGT 68 0.11466729621260666 TruSeq Adapter, Index 1 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCG 68 0.11466729621260666 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAG 68 0.11466729621260666 No Hit CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT 66 0.11129472867694176 Illumina PCR Primer Index 1 (95% over 21bp) CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT 65 0.10960844490910931 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGCCCCAACACGATCTCGTATGCCGT 65 0.10960844490910931 Illumina PCR Primer Index 1 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGCCCCAACACGATCTCGTATGCC 65 0.10960844490910931 No Hit TCTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT 62 0.10454959360561196 Illumina PCR Primer Index 1 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013490270142659607 0.0 2 0.0 0.0 0.0 0.028666824053151664 0.0 3 0.0 0.0 0.0 0.028666824053151664 0.0 4 0.0 0.0 0.0 0.05564736433847088 0.0 5 0.0 0.0 0.0 0.0994907423021146 0.0 6 0.0 0.0 0.0 0.0994907423021146 0.0 7 0.0 0.0 0.0 0.0994907423021146 0.0 8 0.0 0.0 0.0 0.10792216114127685 0.0 9 0.0 0.0 0.0 0.12141243128393646 0.0 10 0.0 0.0 0.0 0.13321641765876363 0.0 11 0.0 0.0 0.0 0.14670668780142324 0.0 12 0.0 0.0 0.0 0.14670668780142324 0.0 13 0.0 0.0 0.0 0.14670668780142324 0.0 14 0.0 0.0 0.0 0.15513810664058547 0.0 15 0.0 0.0 0.0 0.15851067417625037 0.0 16 0.0 0.0 0.0 0.18043236315807223 0.0 17 0.0 0.0 0.0 0.18043236315807223 0.0 18 0.0 0.0 0.0 0.18380493069373713 0.0 19 0.0 0.0 0.0 0.2023540521398941 0.0 20 0.0 0.0 0.0 0.23945229503220802 0.0 21 0.0 0.0 0.0 0.2647465515496948 0.0 22 0.0 0.0 0.0 0.2883545242993491 0.0 23 0.0 0.0 0.0 0.291727091835014 0.0 24 0.0 0.0 0.0 0.291727091835014 0.0 25 0.0 0.0 0.0 0.29678594313851137 0.0 26 0.0 0.0 0.0 0.2984722269063438 0.0 27 0.0 0.0 0.0 0.3507470237091498 0.0 28 0.0 0.0 0.0 0.41651209065461536 0.0 29 0.0 0.0 0.0 0.4671006036895889 0.0 30 0.0 0.0 0.0 0.531179386867222 0.0 31 0.0 0.0 0.0 0.5901993187413578 0.0 32 0.0 0.0 0.0 0.7014940474182996 0.0 33 0.0 0.0 0.0 0.7908670871134195 0.0 34 0.0 0.0 0.0 0.9358874911470102 0.0 35 0.0 0.0 0.0 1.0674176250379415 0.0 36 0.0 0.0 0.0 1.3456544467302958 0.0 37 0.0 0.0 0.0 1.8869515362045124 0.0 38 0.0 0.0 0.0 2.360797274965431 0.0 39 0.0 0.0 0.0 2.8262115948871878 0.0 40 0.0 0.0 0.0 3.411352062325048 0.0 41 0.0 0.0 0.0 3.864962395871977 0.0 42 0.0 0.0 0.0 4.431553741863681 0.0 43 0.0 0.0 0.0 4.994772520319719 0.0 44 0.0 0.0 0.0 5.568109001382752 0.0 45 0.0 0.0 0.0 6.367407507335335 0.0 46 0.0 0.0 0.0 6.85305723247108 0.0 47 0.0 0.0 0.0 7.404472024552292 0.0 48 0.0 0.0 0.0 7.785572156082425 0.0 49 0.0 0.0 0.0 8.22231965195103 0.0 50 0.0 0.0 0.0 8.620282621159488 0.0 51 0.0 0.0 0.0 9.040167279349768 0.0 52 0.0 0.0 0.0 9.507267883039358 0.0 53 0.0 0.0 0.0 10.013153013389093 0.0 54 0.0 0.0 0.0 10.358841185794745 0.0 55 0.0 0.0 0.0 10.707901925736063 0.0 56 0.0 0.0 0.0 11.046844963070386 0.0 57 0.0 0.0 0.0 11.363866311422886 0.0 58 0.0 0.0 0.0 11.657279687025733 0.0 59 0.0 0.0 0.0 11.994536440592222 0.0 60 0.0 0.0 0.0 12.223871033017437 0.0 61 0.0 0.0 0.0 12.448146774139152 0.0 62 0.0 0.0 0.0 12.717952176992345 0.0 63 0.0 0.0 0.0 12.999561566220363 0.0 64 0.0 0.0 0.0 13.208660753431587 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGATC 25 2.7968235E-6 70.04642 33 AAAACGA 20 7.841033E-5 70.04642 31 GATATGC 15 0.0022086627 70.04641 22 CTTTGCA 15 0.0022086627 70.04641 29 TCCGAGA 55 0.0 69.98736 13 ACTGCTT 30 1.0088843E-7 69.98735 53 GCCTAAT 15 0.002216073 69.98735 70 CCGTCTA 25 2.8108989E-6 69.98735 47 AAGGGCG 15 0.002216073 69.98735 8 AGTATGC 15 0.002216073 69.98735 41 TTCCTAT 15 0.002216073 69.98735 5 TCAAAAA 15 0.002216073 69.98735 59 CCTTATA 30 1.0088843E-7 69.98735 1 AATGCCG 30 1.0088843E-7 69.98735 43 TCTTTAT 90 0.0 69.98735 1 GACCACG 40 1.3096724E-10 69.98735 18 CTACTGC 30 1.0088843E-7 69.98735 51 CGTCTAC 25 2.8108989E-6 69.98735 48 TCTTAAT 40 1.3096724E-10 69.98735 1 CTTTATA 95 0.0 66.30381 2 >>END_MODULE