FastQCFastQC Report
Wed 25 May 2016
SRR1780021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66847
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4200562.83752449623768No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG13051.9522192469370352No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1630.24384041168638831No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1580.2363606444567445No Hit
TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT1480.22140110999745688No Hit
CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG1130.16904273938995018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAG150.002215717270.064
GTCACCT150.002215717270.039
CTGAGAG150.002215717270.024
TCTGGCG150.002215717270.011
TAATGCA150.002215717270.062
ATGTGTA150.002215717270.025
CTGGCGC207.874164E-570.012
AAGACGA150.002215717270.029
CTGTGCC150.002215717270.036
AGATGTG150.002215717270.023
CCGGGCG150.002215717270.09
ACAGGGC150.002215717270.012
ACGGTGG150.002215717270.045
TTGTGCG252.8117884E-670.02
TCTTTAT150.002215717270.01
TTTATAC150.002215717270.03
TGTGCGC207.874164E-570.03
TAGATAT207.874164E-570.039
CGTCTGG207.874164E-570.09
GAGATGT150.002215717270.022
Produced by FastQC (version 0.11.5)