FastQCFastQC Report
Wed 25 May 2016
SRR1780021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66847
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT2667639.90605412359567No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG45716.838003201340374No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC25363.793737938875342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23923.5783206426616005No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT12801.9148204107888163TruSeq Adapter, Index 25 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC10601.5857106526844884No Hit
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC4590.686642631681302No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT4110.6148368662767215No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGACGT3140.4697293820216315No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT3030.45327389411641505No Hit
TATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT2140.32013403742875524RNA PCR Primer, Index 25 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC2110.3156461770909689No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG1900.28423115472646493No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTAAGCCGT1770.264783759929391No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC1770.264783759929391No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC1440.2154172962137418No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAG1190.17801846006552274No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCAT1190.17801846006552274No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG1140.17053869283587894No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGATATCTCGTATGCCGT1080.16156297216030638No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGA1030.15408320493066258No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGAAATCTCGTATGCCGT1020.1525872514847338No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG940.1406196239173037No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT930.13912367047137492No Hit
CTTATACACATCTCCGAGCCCACGAGACATGAAGATATCTCGTATGCCGT910.1361317635795174No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTAGTATGCCGT910.1361317635795174No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGAATGCCGT720.1077086481068709No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT710.10621269466094216No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAGG150.002215717270.014
ACTCGTA207.874164E-570.038
ACGTATG252.8117884E-670.040
CATTCTC150.002215717270.020
CTCTATA207.874164E-570.01
CGAGGGC150.002215717270.036
TCTCGCC150.002215717270.023
TCGCCCG150.002215717270.025
TCATGCG150.002215717270.038
CACCATA150.002215717270.011
AACAGGC150.002215717270.047
GCTCTCT150.002215717270.017
ATTCTCG150.002215717270.021
TCTTTAT1200.070.01
CACAGAC150.002215717270.05
AGATATC207.874164E-570.033
GCGAGGG150.002215717270.035
AGGGGGG29600.062.3141970
GAATGCC401.0468284E-861.2542
TCTCGAA401.0468284E-861.2538
Produced by FastQC (version 0.11.5)