FastQCFastQC Report
Wed 25 May 2016
SRR1780020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156399
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3517522.490553008650952No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG8640.5524331997007654No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATT150.002218765270.0201616
AGCCGGT150.002221582769.99776570
ACGGAGA150.002221582769.99776513
CGCGTCT150.002221582769.9977657
CACGGAG200.006941741352.4983212
GTGATAA200.006941741352.498321
CGTTAAC200.006941741352.4983212
GTACTTA200.006941741352.498321
CCGCGTT200.006941741352.4983245
CGCAAGG200.006941741352.4983210
GCGTCCG200.006941741352.4983246
GCCGTAT2100.048.3317954
CTTACCG305.86467E-446.6651769
CGCCGTA2200.046.13488853
TCGCCGT2250.045.1096752
TCGGTGG2250.045.1096745
CTCGGTG2150.043.9520844
GCGCTGG404.5687488E-543.762615
CCGTATC2350.043.19010555
GTCGCCG2400.042.29031851
Produced by FastQC (version 0.11.5)