FastQCFastQC Report
Wed 25 May 2016
SRR1780020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156399
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT2130213.620291689844564No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG38142.4386345181235174No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC25891.6553814282700017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16521.0562727383167412No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT11890.7602350398659837Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC6940.4437368525374203No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5700.3644524581359216No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC3670.234656231817339No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCAAAATCTCGTATGCCGT2110.13491134853803413No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT2020.12915683604115116Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT1900.12148415271197387No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1770.11317207910536513No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC1760.11253268882793369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC150.002219810670.0118448
GGGTCAA207.9131525E-569.989451
GTGCTAG150.002222629569.989451
ATGGTTG150.002222629569.9894546
AGGGGGG23900.062.10254770
CGTCTTA401.0577423E-861.26035748
AGGACCC252.3864085E-455.9915585
TCTTTAT1250.055.9915541
TACCAGT200.00693622252.50887728
GAGTCCA200.00693622252.50887758
AGAGTCC200.00693622252.50887757
ACGATAC200.00693622252.50887727
GACGATA200.00693622252.50887726
CACGTCG200.00693622252.50887723
AAGGCGT200.00693622252.50887723
CGTTTAC200.00693622252.50887728
CCGTTTA200.00693622252.50887727
GCCGTTT200.00693622252.50887726
AGCCGTC407.4501986E-752.49208545
AATCACG407.4501986E-752.49208536
Produced by FastQC (version 0.11.5)