FastQCFastQC Report
Wed 25 May 2016
SRR1780019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326040
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3722711.417924181082077No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG8510.26101091890565575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGG150.002223316370.0012315
CACGCTT150.002223316370.0012354
GCACTAG207.919423E-570.001231
GACCATC200.00694289652.50897633
TTAGACC200.0069471152.5009254
TAAGTAC200.0069471152.500926
CTGCGGA200.0069471152.5009259
ACAGGTA200.0069471152.500921
GCAATCT200.0069471152.5009241
GACATCG451.6818467E-646.66748422
GTGCGCG305.872665E-446.6674844
TCGGTAG305.872665E-446.66748456
TCGTACT404.587054E-543.75076726
GGAATAT350.001254337640.00071
AAGTTGG350.001254337640.000740
ATGCTGC350.001255284239.99456851
ATCGTAC459.186449E-538.88957225
CATCGTA459.186449E-538.88957224
GTTAAGC400.00241669235.00061456
CCAAGTA601.2137336E-535.0006142
Produced by FastQC (version 0.11.5)