FastQCFastQC Report
Wed 25 May 2016
SRR1780019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326040
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT195836.006318243160348No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG32801.0060115323273218No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC22060.6766040976567292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16810.5155809103177524No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC6490.199055330634278No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT6280.19261440314071893Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT5310.16286345233713653No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT4930.15120844068212488No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT4330.13280579070052756No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT4100.12575144154091522No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3380.1036682615629984No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTAAGCCGT3360.10305483989694515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA150.002221576970.0150458
GCGTACT150.002221576970.0150460
TGCGTAC150.002221576970.0150459
CCTTAGT150.002222929470.00429566
AAGCGTG150.002225636369.9828214
CACGTAT850.065.886439
AAGCTTG1000.062.994254
AGGGGGG22500.059.74616670
GCCGACA308.3636405E-658.3369146
CGTATGA1550.054.19687341
ACGTATG1050.053.33660540
GTCTTAA1050.053.32842649
GTACTTA200.006941690652.5112862
CGTACTT200.006941690652.5112861
CGGAACC200.00694590552.50322327
GGTACGG200.00694590552.5032231
AATCTCG200.00694590552.50322336
CGGTATG200.00694590552.50322343
GTGGGAT200.006950120452.49516747
CCGCAAT200.006950120452.49516730
Produced by FastQC (version 0.11.5)