FastQCFastQC Report
Wed 25 May 2016
SRR1780018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87069
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4806655.20449298831961No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG63597.303403048157209No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT7350.8441580815215519No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6330.7270096130654997No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG6250.7178214978924761No Hit
TCTTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1980.22740585053233642No Hit
CTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG1300.14930687156163502No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG1260.14471281397512317No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1070.1228910404391919No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTT150.00221798470.049
TTGTGCG150.00221798470.02
TCTTCCC150.00221798470.034
GCTATAA150.00221798470.061
TGTGCGC150.00221798470.03
AACATCT150.00221798470.07
TCTTTAT252.8189806E-670.01
CAATCTT150.00221798470.07
TACACTG150.00221798470.030
GCACTCT150.00221798470.033
TTTATAC308.315479E-658.3333323
CTTTATA308.315479E-658.3333322
TTAATAC252.3787386E-455.9999962
GGGGCAT200.006930579452.532
GAGCTGC200.006930579452.545
TACCATA200.006930579452.539
TCCTCAT200.006930579452.511
CACTGTG200.006930579452.532
TCGGAGG200.006930579452.529
GTTCGAT200.006930579452.515
Produced by FastQC (version 0.11.5)