FastQCFastQC Report
Wed 25 May 2016
SRR1780018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87069
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3371238.718717339121845No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1020211.717143874398465No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG36114.14728548622357No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC34123.918731121294605No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT9451.0853461048134239TruSeq Adapter, Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC7140.8200392791923647No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC4340.49845524813653536No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4090.46974238822083636No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2870.32962363183222504No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2770.3181384878659454No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG2330.26760385441431506No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG2010.2308513937222203No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT1990.2285543649289644No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1780.20443556259977716No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT1750.20099001940989328No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT1670.19180190423686963RNA PCR Primer, Index 11 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1440.1653860731144265No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT1390.1596435011312867No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT1210.13897024199198338No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1190.13667321319872744No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC1130.12978212681895968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA207.8875884E-570.048
TACTTGT150.00221798470.045
GACAAGT150.00221798470.028
GAATCAG150.00221798470.026
GACCACG150.00221798470.018
GAGACCA150.00221798470.016
TCTTTAT800.070.01
ATAACTC150.00221798470.035
TTCGCAT207.8875884E-570.049
TATAACT207.8875884E-570.034
TGGTCAA150.00221798470.011
AGAGCCC252.8189806E-670.015
AGGGGGG39650.065.5863770
TAAGCCG453.783498E-1062.22221843
CAGAGCC352.9438888E-760.014
GCTATAA308.315479E-658.33333232
TCTGCAT800.056.87553
GCATGAA1050.056.6666756
AAGGGGG46400.056.04525869
GTATTCT252.3787386E-455.99999649
Produced by FastQC (version 0.11.5)