FastQCFastQC Report
Wed 25 May 2016
SRR1780017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389999
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5211913.363880420206206No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG57101.4641063182213288No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7010.1797440506257708No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG6880.17641070874540704No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT6110.1566670683770984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTA150.002223492170.0024351
AAATCGA150.002224623569.99345420
TGCGCGT150.002224623569.9934545
ATGCGCG150.002224623569.9934544
GTCAGAC200.006947652452.50182749
ACGGAGT200.006947652452.50182724
CGGACCT200.006947652452.50182730
CGGTGGT8800.047.33119246
TGGTCGC8750.047.20164549
GCCGTAT8550.047.07162554
TACCCGC305.877306E-446.66230411
CGTGCCG305.877306E-446.66230410
GCATATA305.877306E-446.6623041
TCGCCGT8650.046.53341352
GTCGCCG8850.046.27279751
TCGGTGG9050.046.023745
GATCTCG9000.045.50158341
CTCGGTG9100.045.00156444
CGCCGTA9050.044.8576953
CGGACTT551.3092722E-744.54700539
Produced by FastQC (version 0.11.5)