FastQCFastQC Report
Wed 25 May 2016
SRR1780017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389999
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT358419.190023564162985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98412.5233398034353933No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC52351.3423111341311131No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG37100.9512844904730524No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT11610.29769307100787434TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC6910.17717994148702945No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4800.12307723865958632No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC4290.11000028205200527No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT150.002223928569.9989739
GAACACG150.002223928569.9989718
CCGTTAG150.002223928569.9989738
AGGGGGG44700.062.2474170
ATCGGAT352.9711373E-759.9991232
AAGCCGT252.3892443E-455.9991844
AAGGGGG51100.054.5882469
CCGTCTT53700.053.5094647
TATGCCG54050.053.48672543
GCCGTCT53800.053.4750546
CGTCTTC54050.053.29246548
CGTATGC54450.053.28663641
ATGCCGT54400.053.14260544
TGCCGTC54300.053.11155745
TCGTATG54900.052.91361640
CTCGTAT54400.052.69224539
GTATGCC54800.052.69083842
CTGCTTG54350.052.61192354
CGGAACT200.006949012652.49923334
CGTTAGC200.006949012652.49923339