FastQCFastQC Report
Wed 25 May 2016
SRR1780016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89003
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4986456.02507780636608No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG66027.417727492331719No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT8740.9819893711447928No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG7890.8864869723492467No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6540.7348066919092615No Hit
TCTTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1930.21684662314753436No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG1630.18313989416087098No Hit
CTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG1290.1449389346426525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC150.002218147770.0000116
TAGAACT150.002218147770.0000139
GAGGATG150.002218147770.0000110
TCGATCG150.002218147770.0000117
GATCGCC150.002218147770.0000125
CGAGGAT150.002218147770.000019
TGCTAAG150.002218147770.000011
AGACCCA150.002218147770.0000110
AGATCGC150.002218147770.0000124
CCGATAT150.002218147770.0000120
TGCGGGC150.002218147770.0000119
CGATCGT150.002218147770.0000118
CTTATGC207.88856E-570.01
GCTGGCG308.317005E-658.33333617
GCTAAGG252.37903E-455.9999962
TGGCGGC252.37903E-455.99999619
GGGTGAT252.37903E-455.9999961
AAAAAGG3700.055.8108170
TTATGCG200.006931088452.52
AAACCGA200.006931088452.517
Produced by FastQC (version 0.11.5)