FastQCFastQC Report
Wed 25 May 2016
SRR1780016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89003
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3427638.51106142489579No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1152312.9467546037774No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC41444.656022830691101No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG37384.199858431738257No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT9611.0797388852061167TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC6280.7055941934541532No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5350.6011033335954967No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC4170.4685235329146209No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT4020.45167016842128915No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3900.4381874768266238No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG2030.2280821994764221No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGCATCTCGTATGCCGT1980.22246441131197828No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT1650.18538700942664854No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC1540.17302787546487197No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC1460.16403941440176173No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC1460.16403941440176173No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT1430.1606687415030954No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC1370.15392739570576272No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG1170.13145624304798714No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATATCGTATGCCGT1100.12359133961776571No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGACTCTCGTATGCCGT1050.1179735514533218No Hit
CTTATACACATCTCCGAGACCACGAGACGGGAATGAATCTCGTATGCCGT1030.11572643618754425No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCAGT980.11010864802310034No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAGC150.002218147770.0000116
TCGTGAG150.002218147770.0000147
CTGAATG150.002218147770.0000154
GCAGCTA150.002218147770.0000119
AAAGGGC150.002218147770.0000168
AATGGGG150.002218147770.0000169
TCTGAAT150.002218147770.0000153
CGTAAGA150.002218147770.0000141
AAAATGG150.002218147770.0000167
TCCCGTC150.002218147770.0000145
CGTGAGG150.002218147770.0000148
TCATTCC150.002218147770.000014
CTATCTT150.002218147770.0000149
AATCACG207.88856E-570.036
CACGTAT207.88856E-570.039
ACGTATG207.88856E-570.040
GTATTCT252.8195009E-670.049
CCGTCTA353.6616257E-970.047
CTCTATA207.88856E-570.01
TCACGTA207.88856E-570.038
Produced by FastQC (version 0.11.5)