FastQCFastQC Report
Wed 25 May 2016
SRR1780015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69850
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4581565.59055118110237No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG46016.586972083035075No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG5710.8174659985683609No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT5080.7272727272727273No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC4590.6571224051539012No Hit
TCTTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1480.21188260558339297No Hit
CTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG1050.15032211882605584No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG980.14030064423765212No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGGT150.002216142670.010
ACCGGGA150.002216142670.024
AAACCGA150.002216142670.036
CCGTGCA150.002216142670.02
AACCGAC150.002216142670.037
CTTTACA150.002216142670.01
ACAGACC150.002216142670.042
TCGGCTA150.002216142670.028
AATCAGG150.002216142670.08
TGTTCGG150.002216142670.025
ACCGTGC150.002216142670.01
TAGCACA150.002216142670.015
CGAGGGG150.002216142670.033
ACCGACA150.002216142670.038
CCCTGTT150.002216142670.022
GGTAGCA150.002216142670.013
GGCTATT150.002216142670.030
GCCATAA150.002216142670.01
CCATAAG150.002216142670.02
CGTGCAA150.002216142670.03
Produced by FastQC (version 0.11.5)