FastQCFastQC Report
Wed 25 May 2016
SRR1780015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69850
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3194245.7294201861131Illumina PCR Primer Index 6 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG787811.278453829634932No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG34004.867573371510379No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC29674.247673586256264No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT9541.3657838224767358Illumina PCR Primer Index 6 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC5760.8246241947029349No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT4080.5841088045812455Illumina PCR Primer Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC3700.5297065139584825Illumina PCR Primer Index 6 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3340.4781675017895491Illumina PCR Primer Index 6 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3300.47244094488188976Illumina PCR Primer Index 6 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG2010.2877594846098783No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC1760.25196850393700787Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT1690.24194702934860415TruSeq Adapter, Index 6 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC1650.23622047244094488Illumina PCR Primer Index 6 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT1600.2290622763063708Illumina PCR Primer Index 6 (95% over 23bp)
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG1420.20329277022190406No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT1400.20042949176807445No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC1310.18754473872584107No Hit
CTCTTATACACATCTCCGAGCCCACGAGCCCGGCCAATATCTCGTATGCC1270.18181818181818182No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGGCCAATATCTCGTATGCCGT1080.15461703650680028Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT1000.14316392269148173No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG850.12168933428775949No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT770.11023622047244094No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGACAATATCTCGTATGCCGT700.10021474588403723No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC150.002210927270.0415421
GGGCACG150.002210927270.0415419
GCACGCA150.002210927270.0415421
CACCAAC150.002210927270.0415426
GCACCAA150.002210927270.0415425
TCGTAAG455.456968E-1269.9914240
CTCGAAT150.002217221569.9914139
TGCACGG150.002217221569.9914148
TTGCACG150.002217221569.9914147
CACACCT150.002217221569.9914140
TAAGCAG207.8814715E-569.9914143
TCGAATG150.002217221569.9914140
TAGCCGA150.002217221569.991419
ACGTATG207.8814715E-569.9914140
GCGGCAT150.002217221569.991411
AGGGCAC150.002217221569.9914118
ACACATA252.8151899E-669.991416
GATTGCA150.002217221569.9914145
AGCAGTC150.002217221569.9914145
AGTATGC150.002217221569.9914141
Produced by FastQC (version 0.11.5)