FastQCFastQC Report
Wed 25 May 2016
SRR1780014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351550
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5497315.637320438060021No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG64821.8438344474470205No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG8620.24519982932726497No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC8000.2275636467074385No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT7060.20082491821931447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTT150.00222359870.00014515
GTAACGG150.00222359870.0001453
AGGTCCA150.00222359870.00014543
CCCGAGA252.3887222E-456.0001142
TAGTCCC252.390402E-455.9921567
TATACCG452.696288E-854.4445536
GGGTCTA200.006947986352.500111
TGTCTTG200.006947986352.500115
TAGCGAA200.006951896552.4926452
CTTTATA506.184928E-849.00012
GCGAAGT305.8737444E-446.66676332
GGTATCA305.8737444E-446.6667631
CCCGTAC305.877865E-446.66012613
GCCGTAT12250.046.57152654
CTCGGTG12600.046.1046544
TCGCCGT12150.046.08407252
TCGGTGG12700.046.0171745
TGGTCGC12650.045.64577549
CGGTGGT12700.045.46606446
GTCGCCG12550.045.45179451
Produced by FastQC (version 0.11.5)