FastQCFastQC Report
Wed 25 May 2016
SRR1780014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351550
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3737110.630351301379605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114513.2572891480585975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC51041.4518560659934576No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG38611.098279049921775No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT11000.31290001422272795RNA PCR Primer, Index 37 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC7030.1999715545441616No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6080.17294837149765327No Hit
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC4870.13852936993315318No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4460.12686673303939697No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4070.11577300526240933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGA150.002224512469.992899
CGTCTAC353.7071004E-969.9928948
CGGTTCC150.002225767869.9829318
AGGGGGG45400.062.67875770
AAGGGGG53300.052.99464869
TGGCGTA200.006939111752.51707524
TGTACCG200.006939111752.51707522
TCGGCAC200.00694692752.50213259
ACTGGTC200.00694692752.5021324
GCGGTCC200.006950836652.49466345
GCGTTTC200.006950836652.4946637
AGCATCG200.006950836652.49466355
TTCGGAG200.006950836652.49466310
GTCTTCA1600.052.49466349
TGCCGTA200.006950836652.49466345
GTCGCGT200.00695474852.48719411
TCGCGTT200.00695474852.48719412
GCCGTCT55850.052.00903746
CGTCTTC55450.052.00553548
TATGCCG56200.051.93422343
Produced by FastQC (version 0.11.5)