FastQCFastQC Report
Wed 25 May 2016
SRR1780013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95742
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5279955.14716634287983No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG53565.5942010820747425No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG6710.7008418457939044No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6520.6809968456894572No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT5640.5890831609951745No Hit
TCTTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1420.1483152639385014No Hit
CTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG1340.13995947442083934No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG960.1002694742119446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC150.0022186670.0000135
GCCTTCA150.0022186670.0000159
CCGGGCT150.0022186670.000013
CGCATTA150.0022186670.0000115
CTATACA150.0022186670.000011
AACCACC150.0022186670.0000111
CGACAAG150.0022186670.0000128
GCGCATT150.0022186670.0000114
GCCATCC150.0022186670.0000142
TCAATAT150.0022186670.0000114
CCATCCC150.0022186670.0000143
GGGCCCT150.0022186670.0000123
GCAGGAA150.0022186670.0000135
TTCGGCG150.0022186670.0000123
GGCGCAT150.0022186670.0000113
TTCTGGG150.0022186670.0000155
ATTTCGG150.0022186670.0000121
AAGTAGA207.891594E-570.020
CCTTCAT200.006932676352.560
TATTTCG200.006932676352.520
Produced by FastQC (version 0.11.5)