FastQCFastQC Report
Wed 25 May 2016
SRR1780013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95742
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3491536.46779887614631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89949.393996365231558No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC41184.30114265421654No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG33613.5104760711077687No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT15141.581333166217543No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT10541.100875268951975TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC6130.6402623717908545No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4120.43032316015959554No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC3970.41465605481397927No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3170.3310981596373587No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3050.31856447536086563No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT2710.28305236991080196No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT2400.2506736855298615No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC2210.23082868542541415No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG1890.19740552735476596No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC1880.19636105366505818No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG1750.18278289569885736No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT1550.1618934219047022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCC1550.1618934219047022No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC1470.15353763238704016No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT1440.1504042113179169No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG1250.13055921121346953No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCG1090.11384763217814543No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG150.0022186670.000013
AACCCGA150.0022186670.0000123
TCACAGA150.0022186670.0000113
AGCCCGG150.0022186670.0000128
TAAACAT252.821127E-670.000015
TGAACCC150.0022186670.0000121
ATCACGT252.821127E-670.0000137
CTAACTA150.0022186670.0000142
AATGCGC150.0022186670.0000117
TGACCGT150.0022186670.0000123
GTGCTTG150.0022186670.0000125
ACCAGCC150.0022186670.0000125
CTCGCTC150.0022186670.0000156
CTACCCA150.0022186670.0000122
GATTGCA150.0022186670.000012
ATGCGCC150.0022186670.0000118
CAATGTT150.0022186670.0000126
CGAGAAC252.821127E-670.0000123
ACGTATG150.0022186670.0000140
GAACCCG150.0022186670.0000122
Produced by FastQC (version 0.11.5)