FastQCFastQC Report
Wed 25 May 2016
SRR1780012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438912
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG436289.940033537474482No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG66811.522173009623797No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC8760.19958442694663164No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG8300.1891039661708953No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT7580.17269976669582968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA200.00694950252.499429
CGTTGTG200.00694950252.499417
TGACGGT200.00694950252.49946
CCGCATG200.00694950252.49949
TCGCCGT12900.048.0227152
GCCGTAT12950.047.56702854
CTCGGTG13100.047.02236644
TCGGTGG13100.047.02236645
CGGTGGT13250.047.0183346
TGGTCGC13250.047.0183349
GTCGCCG13350.046.92830351
TCTCGGT13300.046.3152643
CGTATCA13400.045.96962456
GGTGGTC13800.045.65165747
GTGGTCG13850.045.2341448
CGCCGTA13650.045.1276953
TCATTAA13350.045.0931260
GTATCAT13600.044.77890457
ATCTCGG13850.044.7287342
GATCTCG13900.044.56783741
Produced by FastQC (version 0.11.5)