FastQCFastQC Report
Wed 25 May 2016
SRR1780012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438912
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT294986.720709390492854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115382.628772965879265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC42630.9712653105861767No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG31490.7174558909303004No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT8180.18636993292505105RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC6140.13989136774569844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAT150.002224229769.9980649
ATCGTAT453.8380676E-1062.220539
AGGGGGG36800.060.2972470
CAGCCGT200.006943688752.510511
TGGACTT200.006946818452.50452825
AATCAAG200.006949949552.49854747
GCCGTAT407.4750824E-752.49854746
AAGGGGG42650.052.1908269
CGTCTAC352.085689E-549.9986248
CGTATGC45850.049.84594741
TATGCCG45800.049.67111243
CCGTCTT44350.049.6378647
ATGCCGT45750.049.57239544
GCCGTCT44700.049.56238646
GTATGCC46000.049.5312442
CGTCTTC44550.049.4150248
TGCCGTC45550.049.4058845
TCGTATG46400.049.25510440
CTTGAAA45550.049.1753757
CTGCTTG45250.049.11466254
Produced by FastQC (version 0.11.5)