FastQCFastQC Report
Wed 25 May 2016
SRR1780011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294601
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6037420.493481013302738No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG84762.8771117545425846No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG10940.3713497238638022No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT9400.3190756311078374No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7650.25967325297605914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGTG252.3865071E-456.00760332
TGTTTTA200.006943201652.5071332
GAACTTT200.006943201652.5071320
TACTGTT200.006947864352.4982157
ACGTATT200.006947864352.4982151
CGTTTAC200.006947864352.49821541
TAACATT305.87323E-446.66508558
CATTAAA15600.045.9920361
ATCATTA15350.045.82906359
TATCATT15150.045.74102458
GCCGTAT15400.045.68026754
ATTAAAA16050.045.57477662
CGTATCA15450.045.3059156
GTATCAT15450.045.3059157
TCATTAA15550.045.2396260
CGCCGTA15850.045.04578853
CCGTATC15550.045.0145555
TCTCGGT16050.044.7025343
TAGATCT15850.044.60416439
AGATCTC15800.044.52380840