FastQCFastQC Report
Wed 25 May 2016
SRR1780011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294601
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4252314.434099001700606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140934.783758371492289No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG46271.5705988778042166No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC44801.5207008801735227No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT12980.440595924657418RNA PCR Primer, Index 36 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC7220.24507724006367937No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT6430.2182613093641909No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC5080.17243661766253338No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4500.1527489723388583No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3160.10726372279795385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATA150.002222530570.0057752
AGGGGGG50250.064.9904370
TCTTTAT900.058.3480531
AAGGGGG58000.056.24601469
TCTATAG252.387136E-456.0046252
TCTTCTG61600.054.37948250
GTCTTCT61600.054.32266249
CCGTCTT61150.054.19804847
TTGAAAA62800.054.0092358
CTTGAAA62350.053.94991757
TTCTGCT62000.053.9157452
GCCGTCT61950.053.7241246
TCTGCTT61850.053.70693653
TGAAAAA64400.053.64572559
CTTCTGC62650.053.63570851
TCGTATG63800.053.59250340
CGTCTTC62700.053.53710648
GTATGCC62600.053.38990842
CGTATGC63350.053.31026541
CTCGTAT63350.053.31026539