FastQCFastQC Report
Wed 25 May 2016
SRR1780010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319638
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6160919.274616910379866No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG61671.9293700999255408No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG8200.2565402111138225No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT7720.24152322314618413No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6200.1939694279153292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA150.00222439769.9923321
AGGACGG252.3897097E-455.993875
AGTTCCA200.006941879652.51068527
TCGGGTC200.00695047752.49425545
CCATCGA200.00695047752.49425540
CCGCGTG200.00695047752.49425520
CAGTCGG200.00695047752.49425516
CGGACGG200.00695047752.49425520
ACCGCGT305.8761705E-446.66155619
GCCGTAT11050.043.7124654
CGTATCA11200.043.4395456
CGCCGTA11150.043.3204253
TCGCCGT11250.042.9353552
GTCGCCG11500.042.610751
ATCTCGG11550.042.41959842
TCGGTGG11550.042.41959845
CCGTATC11400.042.3704155
TGGTCGC11550.042.1231949
GTATCAT11750.042.00197257
CTCGGTG11500.041.995444