FastQCFastQC Report
Wed 25 May 2016
SRR1780010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319638
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4197913.133294539447752Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105633.3046759146284237No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG40321.2614269892816248No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC39671.2410914847421146No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT12120.37917894618286935Illumina PCR Primer Index 1 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC8890.2781271313173027No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT7760.242774638810154Illumina PCR Primer Index 1 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC4840.15142129534035378Illumina PCR Primer Index 1 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4830.1511084414243613Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT4560.14266138569256473TruSeq Adapter, Index 1 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4110.1285829594729037Illumina PCR Primer Index 1 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4030.12608012814496397Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA150.002221046570.01893629
TACGAAG150.002223805369.9970339
CGTCCGA150.002223805369.9970342
AGGGGGG50450.062.71290270
CGTCTAC252.3889181E-455.99762748
CCGTCTT61750.053.90054747
GCCGTCT62100.053.82219346
CGTCTTC62300.053.7617648
TATGCCG62600.053.67184443
CTCGTAT63000.053.66438739
CTTGAAA63100.053.5793457
TCTCGTA63350.053.53363838
ATGCCGT62850.053.51403844
TCGTATG63600.053.48829740
TGCCGTC62750.053.4877745
GTCTTCT62650.053.46141449
CGTATGC63050.053.45530341
TCTTCTG62650.053.3496950
ATCTCGT62600.053.28048737
CTTCTGC62600.053.28048751