Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780010_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 319638 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT | 41979 | 13.133294539447752 | Illumina PCR Primer Index 1 (95% over 21bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 10563 | 3.3046759146284237 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG | 4032 | 1.2614269892816248 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC | 3967 | 1.2410914847421146 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT | 1212 | 0.37917894618286935 | Illumina PCR Primer Index 1 (95% over 24bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC | 889 | 0.2781271313173027 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT | 776 | 0.242774638810154 | Illumina PCR Primer Index 1 (95% over 21bp) |
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC | 484 | 0.15142129534035378 | Illumina PCR Primer Index 1 (95% over 22bp) |
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT | 483 | 0.1511084414243613 | Illumina PCR Primer Index 1 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT | 456 | 0.14266138569256473 | TruSeq Adapter, Index 1 (95% over 23bp) |
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT | 411 | 0.1285829594729037 | Illumina PCR Primer Index 1 (95% over 21bp) |
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT | 403 | 0.12608012814496397 | Illumina PCR Primer Index 1 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTCGA | 15 | 0.0022210465 | 70.018936 | 29 |
TACGAAG | 15 | 0.0022238053 | 69.99703 | 39 |
CGTCCGA | 15 | 0.0022238053 | 69.99703 | 42 |
AGGGGGG | 5045 | 0.0 | 62.712902 | 70 |
CGTCTAC | 25 | 2.3889181E-4 | 55.997627 | 48 |
CCGTCTT | 6175 | 0.0 | 53.900547 | 47 |
GCCGTCT | 6210 | 0.0 | 53.822193 | 46 |
CGTCTTC | 6230 | 0.0 | 53.76176 | 48 |
TATGCCG | 6260 | 0.0 | 53.671844 | 43 |
CTCGTAT | 6300 | 0.0 | 53.664387 | 39 |
CTTGAAA | 6310 | 0.0 | 53.57934 | 57 |
TCTCGTA | 6335 | 0.0 | 53.533638 | 38 |
ATGCCGT | 6285 | 0.0 | 53.514038 | 44 |
TCGTATG | 6360 | 0.0 | 53.488297 | 40 |
TGCCGTC | 6275 | 0.0 | 53.48777 | 45 |
GTCTTCT | 6265 | 0.0 | 53.461414 | 49 |
CGTATGC | 6305 | 0.0 | 53.455303 | 41 |
TCTTCTG | 6265 | 0.0 | 53.34969 | 50 |
ATCTCGT | 6260 | 0.0 | 53.280487 | 37 |
CTTCTGC | 6260 | 0.0 | 53.280487 | 51 |