FastQCFastQC Report
Wed 25 May 2016
SRR1780009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452180
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5227011.559555929054802No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG72921.6126321376443007No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG9830.21739130434782608No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT7750.17139192357025962No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7740.1711707727011367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG150.002224084769.999561
CCGGGGT252.389556E-455.9996412
GGTCGCA200.006949497452.499672
GTCGTTG200.006949497452.4996721
CGGTGCA200.006949497452.499679
AAGCTTG200.006949497452.4996738
CGCTTTA200.006949497452.4996767
GGTACTC352.0855268E-549.9996871
ACCGGCA305.875781E-446.66637423
CACGTCC305.875781E-446.66637461
GCCGTAT11900.045.2938354
CGTATCA12250.044.28543556
TGGTCGC12100.044.25591749
GATCCTA404.5905348E-543.749721
TCGGTGG12500.043.39972745
CTCGGTG12350.043.07665344
GTCGCCG12600.043.05528351
CGGTGGT12600.043.05528346
ATCTCGG12550.042.94793742
GTATCAT12550.042.94793757
Produced by FastQC (version 0.11.5)