FastQCFastQC Report
Wed 25 May 2016
SRR1780009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452180
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT356017.8731920916449205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122452.707992392410102No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC47161.0429474987836702No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG37340.825777345304967No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT12160.26891945685346547TruSeq Adapter, Index 25 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC7110.157238267946393No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT5140.11367154672917863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA150.00222510269.99148611
AGGGGGG43750.060.87259770
CAGCGTT252.387E-456.011771
CGTATGA850.053.522941
TTGCAGA200.00694051352.5168429
GCGTATG200.00694051352.5168427
TGCCGTA608.367351E-1152.49361445
ATCAAGA200.00695266852.4936110
AAGGGGG50900.052.04671569
CGTCTTC52250.051.23778548
CCGTCTT52450.051.1091347
GCCGTCT52800.051.10173446
TATGCCG54000.051.00305643
TCGTATG54750.050.87965440
TCTTCTG52100.050.8479450
CTCGTAT54850.050.78689239
CTTCTGC52100.050.713651
TCTCGTA55350.050.64424538
CGTATGC54150.050.60326441
GTCTTCT52350.050.53826549