FastQCFastQC Report
Wed 25 May 2016
SRR1780008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105705
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4927046.61085095312426No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG53215.0338205382905254No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7370.6972234047585261No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG6750.6385696040868455No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT5860.554373019251691No Hit
TCTTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1750.16555508254103402No Hit
CTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG1320.12487583368809421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCT150.002219304270.027
ATAATCC150.002219304270.070
TTGTAAG150.002219304270.02
GTCAGAT150.002219304270.030
GCTTCTT150.002219304270.032
ATTCACC150.002219304270.011
ATTCAAG150.002219304270.016
GTCACAA150.002219304270.09
GTTCATC150.002219304270.03
AGCTCTT150.002219304270.028
ATACACG150.002219304270.04
AAAAAGG3500.053.070
GGAGCTC200.00693467252.526
GATCGCC200.00693467252.522
GCTGGGA200.00693467252.537
GAGTCAC200.00693467252.57
TCACAAG200.00693467252.537
CTATATG200.00693467252.51
TGGGTCG200.00693467252.532
GGACTGC200.00693467252.541
Produced by FastQC (version 0.11.5)