FastQCFastQC Report
Wed 25 May 2016
SRR1780008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105705
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3392932.097819403055674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93568.851047727165225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC41913.9648077195969917No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG32963.118111726029989No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT10691.011305047064945Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC6070.5742396291566151No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT4930.4663923182441701No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC4400.4162527789603141No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT4350.411522633744856No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3500.33111016508206803No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCAAAATCTCGTATGCCGT2290.21664065086798168No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1940.18352963435977485No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1720.16271699541175916No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1680.15893287923939264No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT1550.14663450167920156Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT1340.12676789177427747No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC1220.11541554325717798No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1170.1106853980417199No Hit
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1140.10784731091244501No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC1110.10500922378317015No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAACTCTCGTATGCCGT1090.1031171656969869No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT1090.1031171656969869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAA150.002215259770.032182
AGCCGTC207.8959376E-569.99905445
ACGATAC207.8959376E-569.99905427
AAGCCGT207.8959376E-569.99905444
TCTACGA455.456968E-1269.99905411
CGTAAGC252.823399E-669.99905441
GACGATA207.8959376E-569.99905426
GTAAGCC252.823399E-669.99905442
ACATTCT150.002219423469.9990561
ACGGGGG150.002219423469.9990570
AAAAACG150.002219423469.9990566
TTAAGTC150.002219423469.9990525
ACGTGCG150.002219423469.999059
GCAGGTC150.002219423469.9990522
TCGTAAG301.0153963E-769.9990540
CTCCTTT150.002219423469.9990512
TTTCTTG150.002219423469.9990516
GCTCATT150.002219423469.9990546
AGGGGGG40250.064.5208770
CTACGAG501.4551915E-1162.9991512
Produced by FastQC (version 0.11.5)