FastQCFastQC Report
Wed 25 May 2016
SRR1780007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348167
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5148014.786007864042256No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG57151.6414536702214741No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG7590.21799883389293068No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7010.20134016147423506No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT6630.19042585885508967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTT150.002223435370.001355
CGCATGG200.006947478752.50097337
CGACTAC305.8690336E-446.67423227
GCCGTAT8700.041.43754654
TCGCCGT8700.041.431652
GTCGCCG8900.040.89375751
ATCTCGG8900.040.50636742
CTCGGTG8900.040.5005544
CGTATCA8950.040.28007556
TCGGTGG9050.040.2159645
GATCTCG9000.040.05629341
CGCCGTA9000.040.05629353
CGGTGGT9000.040.05054546
GGTACAT350.001253563440.0064851
TACGGCG350.001253563440.00648531
GCGGCGA350.001253563440.00648518
GTACACC350.001254449440.0007421
TCCGATC350.001254449440.0007463
TCTCGGT9100.040.0007443
CGGCGTG350.001255335939.99499534