FastQCFastQC Report
Wed 25 May 2016
SRR1780007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348167
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT327229.398363429044108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99922.8698871518552878No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC41121.1810424307875245No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG30200.8673998397320826No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT7490.2151266489931556Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC5970.17146943851657395No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT5350.15366189213796827No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT3900.11201521109122921No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT3680.10569640431172397No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT3650.10483474884179145No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGATA150.002221245370.0186842
GGTCCGC150.002223778569.99856643
AGGTCCG150.002223778569.99856642
ACCCGTA150.00222504669.988519
AGGGGGG38800.061.9792870
CGTCTTA3350.058.4978648
TCGTATT308.36889E-658.33213440
TTAACGG308.36889E-658.33213435
CGCGGTA252.3889562E-455.9988543
CCGTCTT43650.054.19499647
GCCGTCT44100.053.96714446
TCTGCTT39550.053.5310353
CTGCTTG39850.053.39148354
TGCCGTC48450.053.3838445
CGTATGC49750.053.3255441
CGTCTTC41100.053.3002548
TATGCCG50400.053.262843
CTCGTAT49700.053.23834239
TCGTATG49900.053.2353840
GTCTTCT39150.053.18411649