FastQCFastQC Report
Wed 25 May 2016
SRR1780006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69676
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3453149.55938917274241No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG1001114.367931568976406No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT12251.7581376657672656No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG10471.5026694988231242No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC10281.4754004248234687Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG2910.4176473965210402No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2080.2985245995751765No Hit
TATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGT1230.17653137378724382No Hit
TCTTAATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG1040.14926229978758826No Hit
CTTTACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG950.13634536999827773No Hit
TTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG850.12199322578793273No Hit
CTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT820.11768758252482922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG150.002216115470.0000116
TGGCAAC150.002216115470.0000134
ATGACTG150.002216115470.0000157
GGCAACG150.002216115470.0000135
ACCAAAA150.002216115470.0000131
GCAACGA150.002216115470.0000136
ACTTCCA150.002216115470.0000113
TTGGCAA150.002216115470.0000133
AGTCCAA150.002216115470.0000116
TTTACAC252.8130526E-670.000012
TCTTTAT352.9352668E-760.01
CTTTACA308.298077E-658.3333361
AAAAAGG5900.054.5762770
TTTATAC452.6546331E-854.4444433
CATGACT200.00692478752.556
ATCCACC200.00692478752.521
AACGAGA200.00692478752.538
CCAAAAG200.00692478752.532
GCTGGGG200.00692478752.517
CCACCTT200.00692478752.523
Produced by FastQC (version 0.11.5)