##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780006_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69676 Sequences flagged as poor quality 0 Sequence length 76 %GC 74 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.642846891325565 32.0 27.0 32.0 27.0 32.0 2 30.389459785291923 32.0 32.0 32.0 27.0 32.0 3 30.15722773982433 32.0 32.0 32.0 27.0 32.0 4 29.228514840117114 32.0 27.0 32.0 22.0 32.0 5 30.175799414432515 32.0 32.0 32.0 27.0 32.0 6 34.60198633675871 37.0 37.0 37.0 32.0 37.0 7 34.459469544749986 37.0 37.0 37.0 27.0 37.0 8 34.420761811814685 37.0 37.0 37.0 27.0 37.0 9 34.78586600838165 37.0 37.0 37.0 32.0 37.0 10-11 34.39425340145818 37.0 37.0 37.0 27.0 37.0 12-13 34.20278862162007 37.0 37.0 37.0 27.0 37.0 14-15 34.1366539410988 37.0 34.5 37.0 27.0 37.0 16-17 34.844638038923016 37.0 37.0 37.0 29.5 37.0 18-19 34.66629111889316 37.0 37.0 37.0 29.5 37.0 20-21 34.931497215684026 37.0 37.0 37.0 29.5 37.0 22-23 35.05379901257248 37.0 37.0 37.0 32.0 37.0 24-25 34.44396922900281 37.0 37.0 37.0 29.5 37.0 26-27 34.90439319134279 37.0 37.0 37.0 32.0 37.0 28-29 34.66893908949997 37.0 37.0 37.0 32.0 37.0 30-31 34.561800332969746 37.0 37.0 37.0 32.0 37.0 32-33 34.630453814799935 37.0 37.0 37.0 29.5 37.0 34-35 34.14835811470233 37.0 34.5 37.0 27.0 37.0 36-37 35.21423445662782 37.0 37.0 37.0 32.0 37.0 38-39 35.52375279866812 37.0 37.0 37.0 34.5 37.0 40-41 35.08089586084161 37.0 37.0 37.0 32.0 37.0 42-43 34.96523910672255 37.0 37.0 37.0 32.0 37.0 44-45 34.901393593202826 37.0 37.0 37.0 32.0 37.0 46-47 35.023336586486025 37.0 37.0 37.0 32.0 37.0 48-49 35.380367701934674 37.0 37.0 37.0 32.0 37.0 50-51 35.606134106435505 37.0 37.0 37.0 34.5 37.0 52-53 35.059791032780296 37.0 37.0 37.0 32.0 37.0 54-55 35.05737987255296 37.0 37.0 37.0 32.0 37.0 56-57 34.9499253688501 37.0 37.0 37.0 29.5 37.0 58-59 34.60601928928182 37.0 37.0 37.0 29.5 37.0 60-61 34.64807824789024 37.0 37.0 37.0 29.5 37.0 62-63 33.99399362764797 37.0 37.0 37.0 24.5 37.0 64-65 33.56148458579712 37.0 34.5 37.0 20.0 37.0 66-67 33.84215511797463 37.0 37.0 37.0 24.5 37.0 68-69 34.24381422584534 37.0 37.0 37.0 27.0 37.0 70-71 34.2315431425455 37.0 37.0 37.0 27.0 37.0 72-73 34.16182760204374 37.0 37.0 37.0 27.0 37.0 74-75 32.82689161260692 37.0 34.5 37.0 20.0 37.0 76 31.695720190596475 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.17805980996348225 11101 2 -0.6819156904439119 11101 3 0.29149594682465363 11101 4 -1.600956404952914 11101 5 -0.8819351396158943 11101 6 -0.8166395592847167 11101 7 -2.205197770504327 11101 8 0.03302058870505675 11101 9 -1.6784600921862491 11101 10-11 -0.19686650600240796 11101 12-13 -0.8720427674890487 11101 14-15 -1.0954325935027711 11101 16-17 0.5691818474975747 11101 18-19 -0.005471085337838133 11101 20-21 -2.6004960154853194 11101 22-23 -0.499770266116613 11101 24-25 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12-13 -0.21945267739895513 21611 14-15 -2.471558719628895 21611 16-17 -1.1518204047546803 21611 18-19 -1.0125093736261306 21611 20-21 -0.7021288983682084 21611 22-23 -1.6715045003508422 21611 24-25 -0.466023196483178 21611 26-27 -0.8362572348080519 21611 28-29 -1.2629048882188911 21611 30-31 -1.434997115453136 21611 32-33 -2.3047852015215966 21611 34-35 -0.28689707502238804 21611 36-37 -2.197235135797115 21611 38-39 -1.3625646371293385 21611 40-41 -0.6175462521638266 21611 42-43 -1.8194347850151829 21611 44-45 -0.7717313503993992 21611 46-47 -1.170105391840167 21611 48-49 -2.660773430281111 21611 50-51 -0.7993453531182624 21611 52-53 -1.8171207296318101 21611 54-55 -0.18816546489346564 21611 56-57 -1.732888586817296 21611 58-59 -0.728191029298408 21611 60-61 -0.8536636182759736 21611 62-63 -1.5512639871363803 21611 64-65 -0.8965703756105299 21611 66-67 -0.8021859830785019 21611 68-69 -1.2713508910376774 21611 70-71 -2.051173645785731 21611 72-73 -1.2401965477979928 21611 74-75 -1.066957867426332 21611 76 -1.312895935482782 21612 1 -0.740327960466928 21612 2 0.904729778554497 21612 3 0.5527572080859144 21612 4 0.09379717215360373 21612 5 0.6125004724667775 21612 6 -0.3354524898623552 21612 7 0.8287184987060598 21612 8 0.11304178637330864 21612 9 0.7380740890538107 21612 10-11 0.689990939678566 21612 12-13 0.6788316363265352 21612 14-15 -0.07449989609948204 21612 16-17 -0.5948351106370282 21612 18-19 0.03957395970720512 21612 20-21 0.653876003592579 21612 22-23 0.7463876565118994 21612 24-25 -0.2583026082478881 21612 26-27 0.06631629460371613 21612 28-29 0.47851668040856055 21612 30-31 -1.5465167232962713 21612 32-33 -0.9212067701490483 21612 34-35 -0.38187256521846535 21612 36-37 0.4804609426342523 21612 38-39 0.7654990883608548 21612 40-41 0.583434139993031 21612 42-43 0.40360443067108775 21612 44-45 0.928023551561381 21612 46-47 0.30232107874807923 21612 48-49 0.013246177562031392 21612 50-51 -0.30118358841237836 21612 52-53 -0.07018445512201055 21612 54-55 -0.1605919354817047 21612 56-57 0.7928467073003489 21612 58-59 0.9740148530545341 21612 60-61 0.5305275581946205 21612 62-63 0.6478781697263685 21612 64-65 -0.6085801795321046 21612 66-67 -0.041769316411837565 21612 68-69 -0.3589734400572837 21612 70-71 -0.5058305085308277 21612 72-73 0.5416661973000458 21612 74-75 0.6248313482599386 21612 76 1.5706824958897663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 11.0 13 30.0 14 47.0 15 71.0 16 143.0 17 247.0 18 307.0 19 309.0 20 303.0 21 320.0 22 358.0 23 449.0 24 524.0 25 605.0 26 722.0 27 776.0 28 880.0 29 1112.0 30 1443.0 31 1896.0 32 2714.0 33 4554.0 34 8169.0 35 13351.0 36 30330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 63.140823238991906 6.047993570239394 5.6346518169814575 25.176531373787242 2 57.6496928641139 6.47712268212871 27.577645100177968 8.295539353579425 3 57.37556690969631 7.702795797692175 27.124117343131065 7.797519949480453 4 56.97636032208523 20.609722840206114 12.904938927243759 9.5089779104649 5 56.61920890981113 12.362937022791206 26.598828865032438 4.419025202365233 6 60.12831737738801 26.356733791678028 7.347389875270915 6.167558955663044 7 59.3633388828291 11.97255870026982 7.064125380331822 21.599977036569264 8 59.37769102703944 26.31609162408864 6.521614329180779 7.784603019691142 9 58.21373213158046 7.887938458005626 4.8682473161490325 29.03008209426488 10-11 59.8836041104541 16.3894310810035 15.036023881967967 8.69094092657443 12-13 59.301624662724606 7.989121074688558 16.47554394626557 16.233710316321257 14-15 69.4220391526494 13.666111717090534 7.622423790114243 9.289425340145819 16-17 68.99362764797061 8.806475687467707 6.129083185027843 16.070813479533843 18-19 61.09034022001995 6.444876606554671 13.993441023028181 18.4713421503972 20-21 69.08387633837586 6.808135028848637 8.15942819416138 15.948560438614118 22-23 69.10226049515609 6.429852888410477 5.603157517043416 18.864729099390026 24-25 61.597031848779295 17.19173855008396 5.471990584588004 15.739239016548733 26-27 69.26836269690338 16.643582475151604 5.701675697011016 8.386379130934014 28-29 69.8165065195083 18.1326291164039 5.828447589181276 6.222416774906531 30-31 69.8450630440555 17.594924899710794 6.713456335622582 5.8465557206111365 32-33 71.21236042139105 16.539885753652726 6.43640382352097 5.811350001435256 34-35 61.2477754176474 16.913284344681095 15.825391813536942 6.013548424134566 36-37 68.43989953354863 9.202726946537496 16.545389307499104 5.8119842124147825 38-39 70.23947068152624 7.123122519716682 17.026315222929476 5.611091575827598 40-41 68.45209903121636 15.142447075708649 9.76318622174381 6.6422676713311795 42-43 61.1814012903411 15.621882692349095 15.813496192847865 7.383219824461939 44-45 59.988805247181574 6.745556185459739 17.8678301555066 15.397808411852084 46-47 67.25127734083472 5.768844365348183 10.44836098513118 16.531517308685917 48-49 71.06392445031288 5.599489063666112 15.971066077271946 7.365520408749067 50-51 77.475027269074 5.82697054940008 9.718554452035134 6.9794477294907855 52-53 61.73619134415971 6.3939261289836455 16.0845634405207 15.785319086335944 54-55 67.70721205597417 6.241119483315393 8.607104413347685 17.444564047362757 56-57 67.4194474345174 7.0369573017581635 7.772515249372085 17.77108001435235 58-59 60.37058831972213 15.145752300030141 16.314067142221518 8.169592238026208 60-61 57.990670972371724 9.062791532113383 18.196627197703624 14.749910297811267 62-63 58.34846321107435 16.816769406310776 16.046760292502995 8.788007090111877 64-65 58.526976016534384 17.32234868600462 17.50606404202489 6.644611255436109 66-67 58.23172700850407 27.88618177903764 7.1111270587391004 6.770964153719186 68-69 58.94725511302475 29.322569070685322 5.114459992823825 6.615715823466092 70-71 59.10310876367081 29.654533972500502 5.046358756494532 6.195998507334156 72-73 60.05898900594195 28.79481585670408 5.026982805637684 6.119212331716279 74-75 64.95654732430552 24.50788319805952 4.957408483856847 5.5781609937781225 76 79.4623378114594 9.728441841772877 5.449822023194397 5.359398323573315 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 1.0 9 2.0 10 2.0 11 1.0 12 1.0 13 1.0 14 3.5 15 7.0 16 6.5 17 5.0 18 4.5 19 15.0 20 29.5 21 33.0 22 30.5 23 34.0 24 65.0 25 90.0 26 99.5 27 118.0 28 159.5 29 192.0 30 181.5 31 189.5 32 229.0 33 250.0 34 257.0 35 340.0 36 430.5 37 445.0 38 471.0 39 535.0 40 643.0 41 1327.5 42 1942.0 43 5555.5 44 7265.0 45 3359.0 46 1357.0 47 1227.0 48 956.5 49 812.0 50 808.0 51 837.0 52 784.0 53 754.0 54 806.0 55 816.0 56 800.0 57 744.0 58 714.0 59 588.5 60 445.5 61 411.5 62 395.0 63 354.0 64 314.0 65 279.0 66 211.5 67 180.0 68 167.0 69 129.0 70 86.5 71 69.0 72 71.5 73 70.0 74 55.5 75 45.0 76 51.5 77 54.5 78 42.5 79 34.0 80 38.5 81 45.5 82 49.0 83 50.0 84 47.5 85 45.0 86 52.5 87 60.0 88 62.5 89 67.5 90 78.0 91 99.5 92 113.0 93 118.0 94 158.0 95 218.5 96 244.0 97 301.0 98 571.5 99 17844.5 100 34904.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0071760721051725125 5 0.0 6 0.0071760721051725125 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.176072105172512E-4 20-21 0.0028704288420690048 22-23 0.0014352144210345024 24-25 0.004305643263103507 26-27 0.005023250473620759 28-29 7.176072105172512E-4 30-31 0.0035880360525862563 32-33 0.0028704288420690048 34-35 0.0 36-37 0.0014352144210345024 38-39 0.0021528216315517537 40-41 0.0014352144210345024 42-43 0.006458464894655261 44-45 7.176072105172512E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.176072105172512E-4 54-55 0.0014352144210345024 56-57 0.0014352144210345024 58-59 0.004305643263103507 60-61 0.0014352144210345024 62-63 0.0021528216315517537 64-65 0.004305643263103507 66-67 0.005023250473620759 68-69 0.0014352144210345024 70-71 0.0028704288420690048 72-73 0.0028704288420690048 74-75 0.0035880360525862563 76 0.0057408576841380095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 69676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.944198863310177 #Duplication Level Percentage of deduplicated Percentage of total 1 69.06530657500831 8.939950628623915 2 5.787781350482315 1.4983638555600207 3 4.701186384299811 1.8255927435558872 4 4.645747865617031 2.4054193696538264 5 4.501607717041801 2.91348527470004 6 3.3817496396496285 2.6264423904931395 7 2.4060317108326865 2.1800907055514096 8 1.9625235613704402 2.032263620184856 9 1.263998225967402 1.4725299959813996 >10 2.1066637099456704 3.6813249899534988 >50 0.0776139261558931 0.7190424249382859 >100 0.044350814946224636 1.0419656696710489 >500 0.0 0.0 >1k 0.033263111209668475 4.736207589413858 >5k 0.0 0.0 >10k+ 0.022175407473112318 63.927320741718816 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 34531 49.55938917274241 No Hit CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG 10011 14.367931568976406 No Hit TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT 1225 1.7581376657672656 No Hit CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG 1047 1.5026694988231242 No Hit ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC 1028 1.4754004248234687 Illumina Single End PCR Primer 1 (95% over 21bp) TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG 291 0.4176473965210402 No Hit CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG 208 0.2985245995751765 No Hit TATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGT 123 0.17653137378724382 No Hit TCTTAATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG 104 0.14926229978758826 No Hit CTTTACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG 95 0.13634536999827773 No Hit TTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG 85 0.12199322578793273 No Hit CTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT 82 0.11768758252482922 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0071760721051725125 0.0 3 0.0 0.0 0.0 0.0071760721051725125 0.0 4 0.0 0.0 0.0 0.020093001894483035 0.0 5 0.0 0.0 0.0 0.041621218210000574 0.0 6 0.0 0.0 0.0 0.041621218210000574 0.0 7 0.0 0.0 0.0 0.041621218210000574 0.0 8 0.0 0.0 0.0 0.041621218210000574 0.0 9 0.0 0.0 0.0 0.041621218210000574 0.0 10 0.0 0.0 0.0 0.04879729031517309 0.0 11 0.0 0.0 0.0 0.054538147999311094 0.0 12 0.0 0.0 0.0 0.054538147999311094 0.0 13 0.0 0.0 0.0 0.06745507778862161 0.0 14 0.0 0.0 0.0 0.07319593547275963 0.0 15 0.0 0.0 0.0 0.07319593547275963 0.0 16 0.0 0.0 0.0 0.09328893736724267 0.0 17 0.0 0.0 0.0 0.10333543831448419 0.0 18 0.0 0.0 0.0 0.12199322578793272 0.0 19 0.0 0.0 0.0 0.13634536999827773 0.0 20 0.0 0.0 0.0 0.14639187094551925 0.0 21 0.0 0.0 0.0 0.20380044778689937 0.0 22 0.0 0.0 0.0 0.24685688041793444 0.0 23 0.0 0.0 0.0 0.25546816694414143 0.0 24 0.0 0.0 0.0 0.2985245995751765 0.0 25 0.0 0.0 0.0 0.3272288879958666 0.0 26 0.0 0.0 0.0 0.3372753889431081 0.0 27 0.0 0.0 0.0 0.3760261783110397 0.0 28 0.0 0.0 0.0 0.3961191802055227 0.0 29 0.0 0.0 0.0 0.4205178253631092 0.0 30 0.0 0.0 0.0 0.4635742579941443 0.0 31 0.0 0.0 0.0 0.47218554452035133 0.0 32 0.0 0.0 0.0 0.5181124059934554 0.0 33 0.0 0.0 0.0 0.5611688386244905 0.0 34 0.0 0.0 0.0 0.6716803490441472 0.0 35 0.0 0.0 0.0 0.8582582237786325 0.0 36 0.0 0.0 0.0 0.9716401630403583 0.0 37 0.0 0.0 0.0 1.3132211952465698 0.0 38 0.0 0.0 0.0 1.633274011137264 0.0 39 0.0 0.0 0.0 2.013605832711407 0.0 40 0.0 0.0 0.0 2.507319593547276 0.0 41 0.0 0.0 0.0 2.8776049141741775 0.0 42 0.0 0.0 0.0 3.4344681095355645 0.0 43 0.0 0.0 0.0 3.969803088581434 0.0 44 0.0 0.0 0.0 4.460646420575234 0.0 45 0.0 0.0 0.0 4.836672598886274 0.0 46 0.0 0.0 0.0 5.274412997301797 0.0 47 0.0 0.0 0.0 5.617429243929043 0.0 48 0.0 0.0 0.0 5.964751133819393 0.0 49 0.0 0.0 0.0 6.382398530340433 0.0 50 0.0 0.0 0.0 6.676617486652506 0.0 51 0.0 0.0 0.0 7.002411160227338 0.0 52 0.0 0.0 0.0 7.345427406854584 0.0 53 0.0 0.0 0.0 7.503300993168379 0.0 54 0.0 0.0 0.0 7.7989551639014865 0.0 55 0.0 0.0 0.0 8.030024685688042 0.0 56 0.0 0.0 0.0 8.318502784315976 0.0 57 0.0 0.0 0.0 8.559618807049773 0.0 58 0.0 0.0 0.0 8.898329410413917 0.0 59 0.0 0.0 0.0 9.067684712095987 0.0 60 0.0 0.0 0.0 9.300189448303577 0.0 61 0.0 0.0 0.0 9.488202537459097 0.0 62 0.0 0.0 0.0 9.707790343877376 0.0 63 0.0 0.0 0.0 9.83983007061255 0.0 64 0.0 0.0 0.0 10.068029163557036 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGGG 15 0.0022161154 70.00001 16 TGGCAAC 15 0.0022161154 70.00001 34 ATGACTG 15 0.0022161154 70.00001 57 GGCAACG 15 0.0022161154 70.00001 35 ACCAAAA 15 0.0022161154 70.00001 31 GCAACGA 15 0.0022161154 70.00001 36 ACTTCCA 15 0.0022161154 70.00001 13 TTGGCAA 15 0.0022161154 70.00001 33 AGTCCAA 15 0.0022161154 70.00001 16 TTTACAC 25 2.8130526E-6 70.00001 2 TCTTTAT 35 2.9352668E-7 60.0 1 CTTTACA 30 8.298077E-6 58.333336 1 AAAAAGG 590 0.0 54.57627 70 TTTATAC 45 2.6546331E-8 54.444443 3 CATGACT 20 0.006924787 52.5 56 ATCCACC 20 0.006924787 52.5 21 AACGAGA 20 0.006924787 52.5 38 CCAAAAG 20 0.006924787 52.5 32 GCTGGGG 20 0.006924787 52.5 17 CCACCTT 20 0.006924787 52.5 23 >>END_MODULE