FastQCFastQC Report
Wed 25 May 2016
SRR1780006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69676
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1738324.94833228084276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1604723.030885814340664No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT55037.897984958952868No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG30774.416154773523164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC18882.709684826913141No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT6370.9142315861989782TruSeq Adapter, Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC5690.816637005568632No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC4640.6659394913600092No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4440.6372352029393191No Hit
ATTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2260.3243584591537976No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC2140.30713588610138354No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT1240.17796658820827832RNA PCR Primer, Index 11 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1180.1693553016820713No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1180.1693553016820713No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC1170.1679200872610368No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT1160.1664848728400023No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT1120.16074401515586428No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT1030.14782708536655376No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT920.13203972673517422No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC900.12916929789310522No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG880.12629886905103624No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAG850.12199322578793273No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT840.12055801136689821No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT820.11768758252482922No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT750.10764108157758769No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAAG150.002211065570.0402327
TCGTTGC150.002211065570.0402323
GATAGCC150.002211065570.0402326
GTTGCCA150.002211065570.0402325
TTGCCAA150.002211065570.0402326
GCCCTTT150.002211065570.0402327
AATCTCG252.8154518E-669.9899536
ATCTTAT353.6507117E-969.989951
TATATCA252.8154518E-669.9899534
CACGTAT252.8154518E-669.9899539
ACAGTCA150.002217378269.9899441
TGGTCGA207.882127E-569.98994435
AGAAGGC150.002217378269.98994432
ACGTCAT150.002217378269.98994447
GAAGCCT150.002217378269.98994460
ACGTATG207.882127E-569.98994440
GTATTCT150.002217378269.98994449
CGAAGCC150.002217378269.98994459
TACACAA150.002217378269.9899445
CCGTATT150.002217378269.98994447
Produced by FastQC (version 0.11.5)