##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780006_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69676 Sequences flagged as poor quality 0 Sequence length 76 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.64600436305184 32.0 27.0 32.0 22.0 32.0 2 30.42934439405247 32.0 32.0 32.0 27.0 32.0 3 30.182085653596648 32.0 32.0 32.0 27.0 32.0 4 31.090145817785178 32.0 32.0 32.0 32.0 32.0 5 29.54256845972788 32.0 27.0 32.0 27.0 32.0 6 35.001550031574716 37.0 37.0 37.0 32.0 37.0 7 35.45559446581319 37.0 37.0 37.0 32.0 37.0 8 34.9735059417877 37.0 37.0 37.0 32.0 37.0 9 35.111674034100695 37.0 37.0 37.0 32.0 37.0 10-11 35.3890076927493 37.0 37.0 37.0 32.0 37.0 12-13 35.626499799069975 37.0 37.0 37.0 32.0 37.0 14-15 35.525015787358626 37.0 37.0 37.0 32.0 37.0 16-17 34.560716746081866 37.0 37.0 37.0 29.5 37.0 18-19 34.84425770710144 37.0 37.0 37.0 27.0 37.0 20-21 35.31497215684023 37.0 37.0 37.0 32.0 37.0 22-23 34.715698375337276 37.0 37.0 37.0 29.5 37.0 24-25 35.34553504793617 37.0 37.0 37.0 32.0 37.0 26-27 35.35517251277341 37.0 37.0 37.0 32.0 37.0 28-29 35.55976232849187 37.0 37.0 37.0 32.0 37.0 30-31 35.17555542798094 37.0 37.0 37.0 29.5 37.0 32-33 35.330293070784776 37.0 37.0 37.0 32.0 37.0 34-35 35.04527383891153 37.0 37.0 37.0 32.0 37.0 36-37 34.96289970721626 37.0 37.0 37.0 32.0 37.0 38-39 34.73657356909122 37.0 37.0 37.0 29.5 37.0 40-41 33.87408863884264 37.0 37.0 37.0 24.5 37.0 42-43 34.24407974051323 37.0 37.0 37.0 27.0 37.0 44-45 34.18283914116769 37.0 37.0 37.0 27.0 37.0 46-47 33.809625983121876 37.0 34.5 37.0 24.5 37.0 48-49 32.620357081347954 37.0 32.0 37.0 17.5 37.0 50-51 33.22435271829612 37.0 34.5 37.0 22.0 37.0 52-53 32.24475429129112 37.0 29.5 37.0 17.5 37.0 54-55 32.12771973132786 37.0 32.0 37.0 13.0 37.0 56-57 32.43499913887135 37.0 32.0 37.0 13.0 37.0 58-59 32.47452494402664 37.0 32.0 37.0 17.5 37.0 60-61 33.3178497617544 37.0 34.5 37.0 20.0 37.0 62-63 34.1370629772088 37.0 37.0 37.0 27.0 37.0 64-65 34.227424077157124 37.0 37.0 37.0 27.0 37.0 66-67 34.09021757850623 37.0 37.0 37.0 27.0 37.0 68-69 33.3203398587749 37.0 32.0 37.0 22.0 37.0 70-71 31.023221769332338 37.0 29.5 37.0 13.0 37.0 72-73 33.26656237441874 37.0 34.5 37.0 20.0 37.0 74-75 34.477754176473965 37.0 37.0 37.0 27.0 37.0 76 34.77963717779436 37.0 37.0 37.0 32.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.7595658025989209 11101 2 0.21708124975893028 11101 3 -0.19313451749572863 11101 4 -0.4355842468935478 11101 5 -0.8792529827290387 11101 6 -0.5357535215100384 11101 7 0.9216941017041336 11101 8 -0.997174425526417 11101 9 -0.18107412572534543 11101 10-11 0.2394943649973058 11101 12-13 0.28923479919127715 11101 14-15 0.42315073823008476 11101 16-17 0.41851497893575385 11101 18-19 -0.09923051827239249 11101 20-21 -0.935791542876899 11101 22-23 0.4326359280946761 11101 24-25 -1.6470196229952236 11101 26-27 0.26613260908332137 11101 28-29 -0.3780139807451448 11101 30-31 -0.10348959731295082 11101 32-33 0.7117553053989525 11101 34-35 0.18796453940925062 11101 36-37 -0.8859954435249549 11101 38-39 -0.5657210047686121 11101 40-41 -0.4638163971696869 11101 42-43 0.019905573534444443 11101 44-45 -0.24370036960583974 11101 46-47 -0.34777673852003943 11101 48-49 -0.34200188752382843 11101 50-51 0.578420920289993 11101 52-53 -0.6112245580305853 11101 54-55 0.2920881489083058 11101 56-57 0.4664915051383218 11101 58-59 0.8294349040749793 11101 60-61 -0.6396140064896088 11101 62-63 -2.016199546341376 11101 64-65 -1.1071441804392208 11101 66-67 -1.4268371165779428 11101 68-69 -0.6659572136587641 11101 70-71 -0.7727441585974155 11101 72-73 -0.014122898938040862 11101 74-75 0.21160314774754596 11101 76 1.169128510431939 11102 1 -1.5433495863827034 11102 2 0.8556912883689662 11102 3 0.2369813126200988 11102 4 -0.6525726638819656 11102 5 -0.4946969981730547 11102 6 0.17621559045907276 11102 7 -1.3169159369059003 11102 8 -2.344664773016767 11102 9 -0.4173675620187822 11102 10-11 -1.243131117628181 11102 12-13 -0.04705863710216818 11102 14-15 -0.2656523505730064 11102 16-17 -0.2455776851569098 11102 18-19 0.4447849257430505 11102 20-21 -0.05779926488462195 11102 22-23 0.12684442230317217 11102 24-25 -0.2354366114122115 11102 26-27 -0.08483264188193829 11102 28-29 0.30499760226643247 11102 30-31 -0.9710571648805129 11102 32-33 -0.2017582081145619 11102 34-35 -1.4108771594324452 11102 36-37 -0.15201860954812219 11102 38-39 -0.6135974526450596 11102 40-41 -0.924820258173547 11102 42-43 0.00368935731823683 11102 44-45 0.2968401709347006 11102 46-47 -0.8095528002961032 11102 48-49 -0.5385269840489215 11102 50-51 -0.43547869360961755 11102 52-53 -0.16218980899583357 11102 54-55 -2.353857797037641 11102 56-57 -0.4435471049002828 11102 58-59 -0.9813759067358276 11102 60-61 -1.016834083709682 11102 62-63 -1.1837671139089352 11102 64-65 -2.188997462292498 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11104 24-25 -0.9747223256979254 11104 26-27 -0.25106640811569747 11104 28-29 0.021556043824872972 11104 30-31 0.4766701078467577 11104 32-33 0.1414236100672568 11104 34-35 0.4176942691389769 11104 36-37 0.8489554164259019 11104 38-39 -0.02333771238532023 11104 40-41 0.37647844312515133 11104 42-43 -1.2985833699544926 11104 44-45 -0.37361437451984614 11104 46-47 -0.6076047483480522 11104 48-49 0.3812782107562711 11104 50-51 -0.6783358364667649 11104 52-53 -0.16251448432051063 11104 54-55 0.971142202962362 11104 56-57 -1.0623782737314542 11104 58-59 0.4618059114459925 11104 60-61 -1.0950808369564342 11104 62-63 -0.6746762048180344 11104 64-65 -0.5042572025522389 11104 66-67 -1.645817460558284 11104 68-69 -1.7221611448626923 11104 70-71 0.15723127137801285 11104 72-73 -0.8872801470952894 11104 74-75 -0.8418366556922621 11104 76 1.3638459551493867 11105 1 -2.2262764156509967 11105 2 0.8096982570100835 11105 3 0.34081406523334223 11105 4 0.03244475772082467 11105 5 -0.9260558831904753 11105 6 -1.0927739565792507 11105 7 0.01200392372127368 11105 8 -0.30633724688436104 11105 9 -0.33862191741947356 11105 10-11 -0.8145596890567504 11105 12-13 -0.6853861632345684 11105 14-15 -0.14474642722806408 11105 16-17 -1.5330341311499467 11105 18-19 0.3378162846280617 11105 20-21 0.9066606654289586 11105 22-23 -0.874549305919821 11105 24-25 -0.13021013057597486 11105 26-27 0.15802450097520904 11105 28-29 0.3948930726497011 11105 30-31 -0.43934984780734254 11105 32-33 -0.5212704032365139 11105 34-35 -0.07011060890980048 11105 36-37 0.1657514252950847 11105 38-39 1.2599217111180039 11105 40-41 -0.2133220003338252 11105 42-43 0.1817381378060361 11105 44-45 -0.39828178028481176 11105 46-47 -2.413037120853595 11105 48-49 -2.396715137359024 11105 50-51 -2.5076041291496907 11105 52-53 -1.49982047101674 11105 54-55 -1.3123943824034896 11105 56-57 -1.7602718435762448 11105 58-59 -0.749668589662658 11105 60-61 -0.8614333868455759 11105 62-63 -0.16913296756747087 11105 64-65 0.13016630077370195 11105 66-67 0.4353909651623411 11105 68-69 1.2542800968224483 11105 70-71 -0.35052926077276325 11105 72-73 0.7587841544568192 11105 74-75 0.22412786758934544 11105 76 1.0550876402935074 11106 1 -0.49334958638270265 11106 2 1.041405574083253 11106 3 0.6798384554772419 11106 4 0.26171305040374904 11106 5 0.19101728754123215 11106 6 0.5297870190305005 11106 7 0.22236977737981078 11106 8 1.3339066555546637 11106 9 0.9540610094097914 11106 10-11 -0.22705968905675178 11106 12-13 -0.5256300656735959 11106 14-15 -0.660295207715869 11106 16-17 0.3901366005573692 11106 18-19 1.3929992114573366 11106 20-21 0.7118435922582336 11106 22-23 1.0464872794460334 11106 24-25 0.9002776743020746 11106 26-27 0.3080245009752076 11106 28-29 0.5067833165521449 11106 30-31 0.24144283511948572 11106 32-33 0.7107417918854395 11106 34-35 0.7426942691389797 11106 36-37 1.2694099618804486 11106 38-39 0.6721168330692251 11106 40-41 1.5037511703978765 11106 42-43 1.0786893573182326 11106 44-45 2.1218401709347035 11106 46-47 2.07080434256104 11106 48-49 1.947187301665366 11106 50-51 0.771664163533238 11106 52-53 0.6806673338613081 11106 54-55 0.8836422029623634 11106 56-57 1.0421671808139976 11106 58-59 0.543624093264178 11106 60-61 1.1367373448617428 11106 62-63 0.7162328860910563 11106 64-65 0.07528825199321432 11106 66-67 0.8860007212598902 11106 68-69 0.703975218773671 11106 70-71 1.4674585441052876 11106 72-73 0.5581743983592631 11106 74-75 1.3024815261259235 11106 76 1.5002095915130198 11107 1 -0.990158097021002 11107 2 0.1797034464236802 11107 3 -0.28399133175679836 11107 4 0.5425641142335387 11107 5 -1.204727393309831 11107 6 -0.05904276820353971 11107 7 -0.06220469070529333 11107 8 0.31794920874614974 11107 9 0.8902312221757498 11107 10-11 -0.4634958592695142 11107 12-13 -0.6181832571629542 11107 14-15 -0.1398164843116092 11107 16-17 -0.6407144632724169 11107 18-19 0.6624141050743546 11107 20-21 -0.43895428008219284 11107 22-23 0.17760430072262778 11107 24-25 -0.5199350916553698 11107 26-27 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-0.014774781226329026 21611 70-71 -0.4971247892280495 21611 72-73 -0.5189089349740712 21611 74-75 -2.128768473874075 21611 76 -1.3122904084869802 21612 1 0.299787668519258 21612 2 0.9531702799656045 21612 3 0.7994462986144981 21612 4 0.17543854059982777 21612 5 0.09689964048240896 21612 6 0.1616497641285406 21612 7 1.0091344832621658 21612 8 -1.3278580503276913 21612 9 -0.06554683372746695 21612 10-11 0.008969722707959704 21612 12-13 0.46358562060091657 21612 14-15 -0.11103050183351115 21612 16-17 0.9519013064397228 21612 18-19 -0.7420498081505116 21612 20-21 0.2679710432386244 21612 22-23 0.4016343382695595 21612 24-25 0.7777286546942292 21612 26-27 0.17763234411246032 21612 28-29 0.1251656694933203 21612 30-31 0.8583545998253683 21612 32-33 0.776673164434456 21612 34-35 0.43730211227622817 21612 36-37 -0.3237272930215127 21612 38-39 -0.12004002967587013 21612 40-41 -0.4550723590138901 21612 42-43 0.1664344553574466 21612 44-45 0.6497813474052876 21612 46-47 -0.48238193194876544 21612 48-49 -0.1037930904915001 21612 50-51 0.9098994576508872 21612 52-53 0.0943928240573868 21612 54-55 0.2971225951192267 21612 56-57 -0.5637151721271749 21612 58-59 0.06764370110730766 21612 60-61 -0.3078704982755056 21612 62-63 0.5632917096204721 21612 64-65 0.9037196245422336 21612 66-67 -0.20664633756363315 21612 68-69 0.501769336420729 21612 70-71 0.1652526617523442 21612 72-73 -0.04035501340545267 21612 74-75 1.1095893692631762 21612 76 0.9168762581796841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 3.0 19 9.0 20 23.0 21 79.0 22 117.0 23 213.0 24 274.0 25 459.0 26 679.0 27 946.0 28 1273.0 29 1800.0 30 2558.0 31 3664.0 32 5173.0 33 8244.0 34 13689.0 35 19190.0 36 11281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.537495514890566 18.560459275206316 9.75816289917474 37.14388231072838 2 29.05716705179912 6.7285749142422455 51.48192269602285 12.732335337935785 3 28.890866295424537 8.266835065158734 52.973764280383485 9.868534359033239 4 27.246110568918997 43.84867099144612 19.988231241747517 8.916987197887364 5 26.990642401974856 18.188472357770248 49.427349446007234 5.3935357942476605 6 30.43072639329439 52.91289308627445 10.96838086489745 5.687999655533708 7 30.021815259199723 15.506056604856767 10.322062116080142 44.15006601986337 8 29.205178253631093 52.71973132786039 6.445547964865951 11.629542453642575 9 29.29559676215627 11.936678339743958 5.202652276250072 53.56507262184971 10-11 29.58922984183483 31.43927433475902 26.581651692166375 12.389844131239775 12-13 29.930679143464033 8.39313393420977 31.557494689706644 30.118692232619555 14-15 30.275848211722835 6.532378437338539 10.868878810494289 52.322894540444345 16-17 52.59558528044089 26.342643090877775 5.432286583615592 15.629485045065733 18-19 54.56189451022605 12.45281664872623 5.781126659490492 27.20416218155723 20-21 32.615965325219584 6.90768700843906 5.796113439347838 54.68023422699352 22-23 30.3992766519318 29.551782536310924 5.81405361961077 34.23488719214651 24-25 52.28680306868662 29.630480181996944 5.853182434711467 12.229534314604967 26-27 54.71313534385003 31.56444227539581 6.644465815953033 7.077956564801126 28-29 32.58165689391602 12.30935959176936 26.0519762009 29.057007313414623 30-31 29.40754297197402 26.12337173000323 12.391000107654214 32.07808519036854 32-33 51.478398162767334 12.607291517152289 7.439356968566098 28.47495335151428 34-35 31.7335017366593 26.9397479691133 29.819588368688464 11.50716192553894 36-37 28.90450083242436 31.695275274125954 32.6080716459039 6.792152247545784 38-39 29.461364027785752 11.849847867271372 32.0009759458063 26.687812159136577 40-41 29.465503136078535 6.691257732550629 32.50326525339802 31.339973877972817 42-43 51.636932527664946 6.244169190360685 30.089130653194207 12.02976762878016 44-45 31.877547505597338 28.061312360066594 32.487513634537 7.5736264997990705 46-47 51.437839544714045 8.614119318793463 13.471985991201441 26.47605514529105 48-49 50.57265055399277 6.266146162236638 8.389545898157184 34.77165738561341 50-51 33.41179172168322 6.574717262759055 30.86859176761008 29.14489924794764 52-53 28.13450829553935 7.395659911590792 52.185113956025035 12.284717836844825 54-55 27.917357388390133 7.438265624663609 34.865479701751745 29.778897285194518 56-57 50.41836500373156 6.660830128021127 13.783081692404844 29.137723175842474 58-59 31.025425735753192 8.566384636913604 47.99888049258326 12.409309134749941 60-61 50.14997990699811 28.230667661748665 14.568861587921237 7.050490843331994 62-63 30.466451381413705 56.06745604592752 5.900251166128453 7.565841406530319 64-65 27.976173388833068 59.30314339026841 5.305009329697144 7.415673891201378 66-67 28.06415730740249 59.409379597402136 5.123255229825254 7.403207865370125 68-69 29.598427004994548 57.70566622653425 5.125150697514209 7.5707560709570005 70-71 49.245077214535854 38.31161375509501 5.078506228830587 7.36480280153855 72-73 78.83900506652505 8.679115295738665 4.977537927174085 7.504341710562197 74-75 81.49346921199943 5.884168221616191 5.2253480694703605 7.397014496914023 76 81.06502081240133 6.021243002727142 5.892062580737764 7.021673604133774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.5 15 3.0 16 12.0 17 21.0 18 17.0 19 19.5 20 23.5 21 21.0 22 42.5 23 52.0 24 57.0 25 74.0 26 85.0 27 95.5 28 110.0 29 125.0 30 162.5 31 206.0 32 236.0 33 260.0 34 269.0 35 286.0 36 367.5 37 441.0 38 525.5 39 627.5 40 685.0 41 860.5 42 996.0 43 1252.0 44 2612.0 45 8389.0 46 13062.0 47 15966.5 48 10234.0 49 1532.0 50 1467.0 51 1153.0 52 826.0 53 794.5 54 776.0 55 793.0 56 840.5 57 810.5 58 750.0 59 651.5 60 524.5 61 460.0 62 424.0 63 376.0 64 287.0 65 248.0 66 236.0 67 222.0 68 186.0 69 132.5 70 91.0 71 67.0 72 62.5 73 55.5 74 40.5 75 28.0 76 28.5 77 26.0 78 20.5 79 18.0 80 17.5 81 14.0 82 9.5 83 8.0 84 6.0 85 4.5 86 4.0 87 3.0 88 5.5 89 6.0 90 2.5 91 1.5 92 2.0 93 2.0 94 3.0 95 9.0 96 14.0 97 22.0 98 157.5 99 8090.0 100 15895.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014352144210345024 2 0.004305643263103507 3 0.0 4 0.0 5 0.0 6 0.004305643263103507 7 0.0 8 0.0 9 0.0 10-11 0.0028704288420690048 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0014352144210345024 20-21 0.0 22-23 0.0 24-25 0.006458464894655261 26-27 0.012916929789310522 28-29 0.013634536999827774 30-31 0.01219932257879327 32-33 0.008611286526207015 34-35 0.0028704288420690048 36-37 0.0 38-39 0.0 40-41 0.004305643263103507 42-43 0.004305643263103507 44-45 0.0 46-47 0.007893679315689765 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0035880360525862563 56-57 0.0 58-59 0.0035880360525862563 60-61 0.0 62-63 0.0014352144210345024 64-65 0.008611286526207015 66-67 0.005023250473620759 68-69 0.0 70-71 0.0 72-73 0.004305643263103507 74-75 0.008611286526207015 76 0.008611286526207015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 69676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.882484643205693 #Duplication Level Percentage of deduplicated Percentage of total 1 64.97326203208557 8.370170503473219 2 8.19964349376114 2.1126356277627876 3 5.570409982174688 2.152821631551754 4 5.158199643493761 2.6580171077558985 5 4.523172905525847 2.91348527470004 6 3.4536541889483066 2.6694988231241745 7 2.3172905525846703 2.0896721970262355 8 1.8270944741532977 1.8830013203972675 9 1.0583778966131907 1.2271083299844996 >10 2.5846702317290555 4.80366266720248 >50 0.1336898395721925 1.2916929789310523 >100 0.12254901960784313 3.0943222917503874 >500 0.022281639928698752 1.7308685917676103 >1k 0.022281639928698752 7.125839600436305 >5k 0.011140819964349376 7.897984958952868 >10k+ 0.022281639928698752 47.97921809518342 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 17383 24.94833228084276 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16047 23.030885814340664 No Hit ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 5503 7.897984958952868 No Hit TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 3077 4.416154773523164 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 1888 2.709684826913141 No Hit ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT 637 0.9142315861989782 TruSeq Adapter, Index 11 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 569 0.816637005568632 No Hit ATCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 464 0.6659394913600092 No Hit CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 444 0.6372352029393191 No Hit ATTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 226 0.3243584591537976 No Hit CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC 214 0.30713588610138354 No Hit TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT 124 0.17796658820827832 RNA PCR Primer, Index 11 (95% over 21bp) CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 118 0.1693553016820713 No Hit TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC 118 0.1693553016820713 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 117 0.1679200872610368 No Hit ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT 116 0.1664848728400023 No Hit ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT 112 0.16074401515586428 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT 103 0.14782708536655376 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT 92 0.13203972673517422 No Hit CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC 90 0.12916929789310522 No Hit CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 88 0.12629886905103624 No Hit TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAG 85 0.12199322578793273 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT 84 0.12055801136689821 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT 82 0.11768758252482922 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT 75 0.10764108157758769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0071760721051725125 0.0 3 0.0 0.0 0.0 0.0071760721051725125 0.0 4 0.0 0.0 0.0 0.020093001894483035 0.0 5 0.0 0.0 0.0 0.04592686147310408 0.0 6 0.0 0.0 0.0 0.04592686147310408 0.0 7 0.0 0.0 0.0 0.04592686147310408 0.0 8 0.0 0.0 0.0 0.04592686147310408 0.0 9 0.0 0.0 0.0 0.04592686147310408 0.0 10 0.0 0.0 0.0 0.05310293357827659 0.0 11 0.0 0.0 0.0 0.0588437912624146 0.0 12 0.0 0.0 0.0 0.0588437912624146 0.0 13 0.0 0.0 0.0 0.07176072105172512 0.0 14 0.0 0.0 0.0 0.07463114989379413 0.0 15 0.0 0.0 0.0 0.07463114989379413 0.0 16 0.0 0.0 0.0 0.09615936620931168 0.0 17 0.0 0.0 0.0 0.10620586715655318 0.0 18 0.0 0.0 0.0 0.12486365463000172 0.0 19 0.0 0.0 0.0 0.14065101326138124 0.0 20 0.0 0.0 0.0 0.14065101326138124 0.0 21 0.0 0.0 0.0 0.17653137378724382 0.0 22 0.0 0.0 0.0 0.17796658820827832 0.0 23 0.0 0.0 0.0 0.18944830357655434 0.0 24 0.0 0.0 0.0 0.19805959010276136 0.0 25 0.0 0.0 0.0 0.21097651989207186 0.0 26 0.0 0.0 0.0 0.22102302083931338 0.0 27 0.0 0.0 0.0 0.26120902462827944 0.0 28 0.0 0.0 0.0 0.2841724553648315 0.0 29 0.0 0.0 0.0 0.32005281589069406 0.0 30 0.0 0.0 0.0 0.36885010620586717 0.0 31 0.0 0.0 0.0 0.3788966071531087 0.0 32 0.0 0.0 0.0 0.42482346862621273 0.0 33 0.0 0.0 0.0 0.48079683104655835 0.0 34 0.0 0.0 0.0 0.6013548424134566 0.0 35 0.0 0.0 0.0 0.7936735748320799 0.0 36 0.0 0.0 0.0 0.9271485159882886 0.0 37 0.0 0.0 0.0 1.362018485561743 0.0 38 0.0 0.0 0.0 1.7150812331362306 0.0 39 0.0 0.0 0.0 2.1011539123945115 0.0 40 0.0 0.0 0.0 2.6034789597565875 0.0 41 0.0 0.0 0.0 3.0010333543831447 0.0 42 0.0 0.0 0.0 3.5722486939548768 0.0 43 0.0 0.0 0.0 4.129111889316264 0.0 44 0.0 0.0 0.0 4.635742579941443 0.0 45 0.0 0.0 0.0 5.047649118778345 0.0 46 0.0 0.0 0.0 5.504047304667317 0.0 47 0.0 0.0 0.0 5.851369194557667 0.0 48 0.0 0.0 0.0 6.223089729605603 0.0 49 0.0 0.0 0.0 6.678052701073541 0.0 50 0.0 0.0 0.0 6.98088294391182 0.0 51 0.0 0.0 0.0 7.332510477065274 0.0 52 0.0 0.0 0.0 7.691314082323899 0.0 53 0.0 0.0 0.0 7.856363740742867 0.0 54 0.0 0.0 0.0 8.183592628738733 0.0 55 0.0 0.0 0.0 8.449107296630116 0.0 56 0.0 0.0 0.0 8.726103679889775 0.0 57 0.0 0.0 0.0 8.960043630518399 0.0 58 0.0 0.0 0.0 9.308800734829784 0.0 59 0.0 0.0 0.0 9.482461679774959 0.0 60 0.0 0.0 0.0 9.70922555829841 0.0 61 0.0 0.0 0.0 9.898673861874965 0.0 62 0.0 0.0 0.0 10.103909524082898 0.0 63 0.0 0.0 0.0 10.251736609449452 0.0 64 0.0 0.0 0.0 10.485676560078076 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCAAG 15 0.0022110655 70.04023 27 TCGTTGC 15 0.0022110655 70.04023 23 GATAGCC 15 0.0022110655 70.04023 26 GTTGCCA 15 0.0022110655 70.04023 25 TTGCCAA 15 0.0022110655 70.04023 26 GCCCTTT 15 0.0022110655 70.04023 27 AATCTCG 25 2.8154518E-6 69.98995 36 ATCTTAT 35 3.6507117E-9 69.98995 1 TATATCA 25 2.8154518E-6 69.98995 34 CACGTAT 25 2.8154518E-6 69.98995 39 ACAGTCA 15 0.0022173782 69.989944 1 TGGTCGA 20 7.882127E-5 69.989944 35 AGAAGGC 15 0.0022173782 69.989944 32 ACGTCAT 15 0.0022173782 69.989944 47 GAAGCCT 15 0.0022173782 69.989944 60 ACGTATG 20 7.882127E-5 69.989944 40 GTATTCT 15 0.0022173782 69.989944 49 CGAAGCC 15 0.0022173782 69.989944 59 TACACAA 15 0.0022173782 69.989944 5 CCGTATT 15 0.0022173782 69.989944 47 >>END_MODULE