FastQCFastQC Report
Wed 25 May 2016
SRR1780005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177273
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3442819.420893198625848No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG89855.068453740840399No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT10410.5872298658002065No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG10220.5765119335713842No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC9130.51502484867972Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG2800.15794847495106418No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG1970.11112803416199872No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAT150.002221792770.01
GATCCGC150.002221792770.02
GGATCCG150.002221792770.01
GATTTCC150.002221792770.050
CGCGAGC150.002221792770.09
CCGTCAG150.002221792770.026
AGCGGCA150.002221792770.021
AGGCCCG150.002221792770.022
CAGATCC150.002221792770.054
CGAGCGG150.002221792770.011
CCGCGAG150.002221792770.08
GCGAGCG150.002221792770.010
ACGACGA308.351008E-658.33333221
CTTTATA553.6379788E-1157.272732
GATCCTA252.385505E-455.9999961
CACTGAG252.385505E-455.9999967
GTATAGA252.385505E-455.9999962
GATCTAA252.385505E-455.9999961
GCGACCC200.006942387752.50000431
CCGATCT200.006942387752.50000431
Produced by FastQC (version 0.11.5)