FastQCFastQC Report
Wed 25 May 2016
SRR1780005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177273
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT170929.64162619236996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149068.408499884359152No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT63283.5696355338940506No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG27951.5766642410293727No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC20261.1428700366102No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT6550.3694866110462394TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC6330.3570763737286558No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC5240.29558928883699154No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4870.2747175260756009No Hit
ATTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2630.14835874611474956No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC2210.12466647487208996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGG150.00221565270.0488428
GACGAAT301.0143776E-770.0488426
ACGAATT252.8193717E-670.0488427
CCGCTCG150.002218134770.0290725
GCCGCTC150.002218134770.0290724
CTCGTTA150.002220619770.00931513
GCGGATC150.002220619770.00931534
ATGCCGC150.002220619770.00931514
TGCCGCT150.002220619770.00931515
CGGAACA150.002223106869.9895655
AGATCGG150.002223106869.989565
CAACGGA150.002223106869.9895652
GGATCCC150.002223106869.9895636
CAGATCG150.002223106869.989564
AGTGACC150.002223106869.9895635
CGGATCC150.002223106869.9895635
ACGGAAC150.002223106869.9895654
GAGCCAG207.916004E-569.989561
GAGTTCC150.002223106869.9895661
CAATAAC150.002223106869.9895640
Produced by FastQC (version 0.11.5)