FastQCFastQC Report
Wed 25 May 2016
SRR1780004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148874
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3710024.920402488009998No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG106267.137579429584749No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG13140.8826255759904349No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT12660.8503835458172684No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC11080.744253529830595Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3190.2142751588591695No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2180.14643255370313152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT207.9067526E-569.998665
TTGGCTG150.002221260269.9986542
TCCGACC150.002221260269.9986545
CGCTGTG150.002221260269.9986541
GGATAGA150.002221260269.9986531
TGTGCGC150.002221260269.998653
GATGGTC150.002221260269.9986515
GAGCGTC150.002221260269.9986528
CTGGGTG150.002221260269.9986544
GTGCGCG150.002221260269.998654
TGCACGG150.002221260269.9986543
TGCCGGC301.01830665E-769.9986519
CGCGTCT401.0588337E-861.2488257
GCGCGTC352.958659E-759.998856
TTTACCG252.3844841E-455.99892417
CTGAGCG200.006940740652.49899326
TTCACAG200.006940740652.49899339
TTCGTTC200.006940740652.49899323
GTTAGTG200.006940740652.49899333
TACGGAG200.006940740652.49899347