FastQCFastQC Report
Wed 25 May 2016
SRR1780004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148874
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1818812.217042599782365No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT1726811.599070354796675No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT68504.601206389295646No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG32312.170291656031275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC22551.5147037091768878No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC6030.40504050405040504No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT5960.4003385413168183TruSeq Adapter, Index 2 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5840.3922780337735266No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC5230.35130378709512744No Hit
ATTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3650.24517377110845415No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC2230.14979109851283634No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA150.002218807670.018151
GTATTCT150.002221767369.9946349
CGATTGG150.002221767369.9946352
AGTGAAG150.002221767369.9946350
GCGATGT150.002221767369.9946331
ACGCTCA150.002221767369.9946359
CGATGTA150.002221767369.9946332
GCCGTAT150.002221767369.9946346
CGTGAAT207.909008E-569.9946228
CGTCTAC207.909008E-569.9946248
AGACGTG207.909008E-569.9946225
CTTTATA750.065.350272
AGGGGGG31500.063.88398770
ATTATAC8500.063.016331
ATATCGT501.4551915E-1162.9951637
TATCGTA501.4551915E-1162.9951638
AAACTCG352.9598414E-759.99539636
CGTCTTA352.9598414E-759.99539648
GAAACTC352.9598414E-759.99539635
CCGACTT352.9598414E-759.99539647
Produced by FastQC (version 0.11.5)