FastQCFastQC Report
Wed 25 May 2016
SRR1780003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106155
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4044038.095238095238095No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG86808.176722716782065No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG10600.9985398709434318No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC10300.9702793085582404Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT9890.9316565399651453No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG2490.23456266779708917No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2020.19028778672695587No Hit
TATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGT1430.13470868070274597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGTC150.002215598270.0296914
TGGTCAT150.002215598270.0296916
ATTTAAT150.002219744769.99670464
TCCATGA150.002219744769.99670424
CGAACTT150.002219744769.99670428
CTTTTGA150.002219744769.9967047
CCGGCAT150.002219744769.99670440
CGGCATG150.002219744769.99670441
GATTTGT150.002219744769.99670461
GTTAGGC150.002219744769.99670466
CCTCGTA150.002219744769.99670443
GCTTGAG150.002219744769.99670449
TTTCAAC150.002219744769.99670412
GACTTGG150.002219744769.99670412
GTTAATC150.002219744769.9967044
CGTAGAT150.002219744769.99670446
CAAGGGT150.002219744769.99670437
TTGAATT150.002219744769.99670450
GTTCCTA150.002219744769.99670435
TCGTAGA150.002219744769.99670445
Produced by FastQC (version 0.11.5)