FastQCFastQC Report
Wed 25 May 2016
SRR1780003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106155
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT1856217.485751966464132Illumina PCR Primer Index 6 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1460613.759125806603551No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT77157.267674626725072Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG33273.1340963685177337No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC21972.0696151853421885No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT8270.7790495030851113Illumina PCR Primer Index 6 (95% over 24bp)
ATCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC7640.7197023220762093No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC6150.5793415288964251No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT4470.42108237953935285Illumina PCR Primer Index 6 (95% over 21bp)
ATTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3270.308040129998587Illumina PCR Primer Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC2130.20064999293485938Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT1620.1526070368800339TruSeq Adapter, Index 6 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC1500.14130281192595734Illumina PCR Primer Index 6 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT1410.13282464321039988No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC1330.12528849324101551No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT1310.1234044557486694Illumina PCR Primer Index 6 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG1300.12246243700249634No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT1280.12057839951015024No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTG150.00221387970.0433830
ACCGGAC301.0109943E-770.0433827
GCTATAT150.00221387970.0433824
ATTCGTA150.00221387970.0433820
GTCAGGC150.00221387970.0433820
GAGACGC207.871343E-570.0433824
CGTAGCA150.00221387970.0433823
TACGAGG150.00221387970.0433824
TCGTAGC150.00221387970.0433822
GCAATAC150.00221387970.0433824
TGAGACG150.00221387970.0433823
AACGGCC252.8128707E-670.0433727
GAACGGC252.8128707E-670.0433726
GCGGTTC150.002218024370.0103759
TTGCTGA207.889757E-570.0103763
GCAACGG150.002218024370.0103734
AGCGGTT150.002218024370.0103758
GTGTACG207.889757E-570.0103768
TGTAGAC150.002218024370.0103734
GCCACTA150.002218024370.0103731
Produced by FastQC (version 0.11.5)