FastQCFastQC Report
Wed 25 May 2016
SRR1780002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308993
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4001312.949484292524428No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG99133.208163291725055No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG16530.5349635752266233No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC13500.43690310136475585Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT10980.35534785577666805No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3570.11553659791645765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGT150.002222789770.0045323
GGTCTAA150.002224217269.99321
TATTCGT200.00694102652.51189832
CTGGATC200.00694102652.51189828
TTCGTAA200.006949919352.494934
GCATTCT200.006949919352.49491
TGTCTTG200.006949919352.49496
CGCAGCT200.006949919352.494918
GATCTAC200.006949919352.49491
GTCGCCG17100.048.3072251
TCGCCGT16950.048.11520852
TGGTCGC17300.047.74875349
GCCGTAT17050.047.62771654
CGCCGTA17100.047.48845753
CGGTGGT17600.047.1337346
CGTATCA17450.046.93713456
GGTGGTC17750.046.7354247
GATCTCG17600.046.72841641
TCGGTGG17900.046.72730645
GTAGATC17700.046.66213638
Produced by FastQC (version 0.11.5)