FastQCFastQC Report
Wed 25 May 2016
SRR1780002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308993
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT194306.288168340383116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175685.685565692426689No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT64542.0887204564504698No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG30190.9770447874223688No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC27910.9032567080807655No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT8300.26861449935759063RNA PCR Primer, Index 37 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC7200.23301498739453647No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6680.2161861271938199No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC6320.20453537782409312No Hit
ATTAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3470.1123002786470891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT301.0221993E-769.9961239
GCCGACA150.0022238569.9961246
AGGGGGG34950.061.48442570
GTGCTTA252.3889472E-455.9968915
CGTCTTA950.055.2600948
AATCTCG653.6379788E-1253.84316636
GACGACG200.006939884352.51408426
CGGGTGG200.006944329552.5055820
GTACTTA200.006944329552.505586
ACGAGGA200.006944329552.505586
ATCCTGG200.006948776552.49708661
GCACGTA200.006948776552.49708652
GACCATC407.4692434E-752.49708633
ATATCGT407.4692434E-752.49708637
TGCCGAC407.4692434E-752.49708645
ATTATAC10900.052.345021
AAGGGGG41150.051.96552369
CCGTCTT41650.049.6611147
GCCGTCT41850.049.59103446
CGTATGC43750.049.27726441