FastQCFastQC Report
Wed 25 May 2016
SRR1780001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176831
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4351524.60824176756338No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG103705.864356362854929No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG14860.8403503910513428No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC12500.7068896290808738Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT10990.6214973618879043No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG2790.15777776521085105No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2440.13798485559658658No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG150.002221783470.035
CCTAAGG150.002221783470.03
CCGTATA150.002221783470.055
ACCAGGG150.002221783470.07
GCATAAG200.006942361652.49999630
TCTTCGT200.006942361652.49999633
GTCCTCA200.006942361652.49999612
TTAGACG200.006942361652.49999639
CCTCAGC200.006942361652.4999961
TGGTCGA200.006942361652.49999649
GGTAGCC200.006942361652.49999650
ACGGTGG200.006942361652.49999645
AAAAAGG9600.049.94791470
TCTCGGT17700.047.0621543
GCCGTAT17850.047.05882354
TCGCCGT17700.046.86440752
TCGGTGG17850.046.86274745
CTCGGTG17800.046.79775244
ATCTCGG18100.046.79557842
GTCGCCG17850.046.66666851