FastQCFastQC Report
Wed 25 May 2016
SRR1780001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176831
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT1996711.291572179086245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174429.86365512834288No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT71674.053022377298098No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG30961.7508242333075081No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC24361.377586509152807No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT8860.5010433690925233TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT7570.42809235937137713No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC7190.4066029146473187No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC6510.36814811882531906No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT5440.3076383665759963No Hit
ATTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3360.19001193229693888No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC2650.14986060136514526No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT2560.14477099603576296No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT2210.12497808642149849No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT1830.10348864169743993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTG150.00221975470.0161332
AGACGTA150.002222246369.996325
AGGGGGG36950.063.93439570
ATTATAC10650.063.095281
ATATCCG352.9625517E-759.9968510
CATCGTT308.339519E-658.3467732
TCGTTGT308.3535215E-658.3302734
ACGTATG308.3535215E-658.3302740
CACGTAT308.3535215E-658.3302739
TCCACGA252.3861072E-455.9970612
AAGGGGG44400.052.89136569
TAAACAC2650.052.827413
TGATGTA200.00693603652.51209630
TTGATCT200.006943801452.49724670
CAGAATG200.006943801452.49724639
TTGCTTT200.006943801452.4972464
CGCATTC200.006943801452.4972468
CCGTCTA200.006943801452.49724647
CGGTGAG200.006943801452.49724634
CGTCCGC200.006943801452.49724645
Produced by FastQC (version 0.11.5)