FastQCFastQC Report
Wed 25 May 2016
SRR1780000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137840
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3655526.51987811955891No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG128549.325304701102729No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG16001.1607661056297156No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT14621.0606500290191527No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC12610.9148287869994196Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3710.2691526407428903No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2870.20821242019733024No Hit
TATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGT1680.12188044109112013No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG150.002220740370.062
GTACATG150.002220740370.01
GTATTCG150.002220740370.025
TAAAGGG150.002220740370.02
TGGACGC150.002220740370.049
GGACTGA150.002220740370.07
GAGAGTT150.002220740370.09
GATCAAG150.002220740370.01
ATGTCGA150.002220740370.038
GTGGATC150.002220740370.016
CTTGGGA150.002220740370.025
CATTAGA150.002220740370.032
TACATGC150.002220740370.02
TTCCCCG150.002220740370.015
GTTGTGC150.002220740370.01
GGTGGAC150.002220740370.047
GATATCT150.002220740370.056
CAGGAAG150.002220740370.05
TAGGGCG150.002220740370.04
AGTTCTC150.002220740370.012
Produced by FastQC (version 0.11.5)