FastQCFastQC Report
Wed 25 May 2016
SRR1780000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137840
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2095715.203859547301219No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1814113.160911201392919No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT57954.204149738827626No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG31702.299767846778874No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC28112.0393209518282065No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC6240.4526987811955891No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT6090.4418165989553105No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT5330.386680208937899RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC5180.3757980266976204No Hit
ATTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3130.2270748694138131No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT2420.1755658734764945No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC2350.1704875217643645No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT1550.1124492164828787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC150.002220740370.051
AGCGTTC150.002220740370.037
GGCAGCG252.8277373E-670.015
CGAACGA150.002220740370.014
TACCACG150.002220740370.025
ACCGCTT150.002220740370.038
CGTCTTA401.3460522E-1070.048
TTGAGCA150.002220740370.036
TAGCAGA150.002220740370.042
GAAAGCG150.002220740370.034
AGCGGAA150.002220740370.018
AAGCCGT301.01759724E-770.044
GTTCGAA150.002220740370.038
CGTAGCG150.002220740370.015
ATCCCAA150.002220740370.069
TTAGTCG150.002220740370.020
GCGGAAT150.002220740370.019
TGAGATA150.002220740370.035
GAGATAT150.002220740370.036
AGGGGGG29500.063.7118770
Produced by FastQC (version 0.11.5)