FastQCFastQC Report
Wed 25 May 2016
SRR1779999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189433
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4325722.834986512381686No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG121736.426018697903745No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG16460.8689087962498614No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT13780.7274339740171987No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC12000.6334693532805794Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3940.2079891043271236No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2430.12827754403931732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGC150.002222261269.9981521
CGACGAG150.002222261269.9981522
GTGTCGG150.002222261269.9981542
TACAGCA150.002222261269.9981522
TCGCGGT150.002222261269.9981552
GTCGGGC150.002222261269.9981510
GCATAGG150.002222261269.998151
TGCCGGC401.0588337E-861.26455719
GGACTAT352.963152E-759.9984176
GCAGTAT308.354518E-658.33179556
CTGCCGG553.45608E-1157.2863418
CTGCGGG252.383123E-456.01330618
TTAATAC252.3862354E-455.998523
GGTCGCA252.3862354E-455.9985252
CACGTCT252.3862354E-455.998527
AGGACAG200.00693659652.51247820
GCCCGCG200.00693659652.51247815
TTGGCTG200.006943844752.49861561
GTCGCGG200.006943844752.49861551
TGCCCAG200.006943844752.49861553
Produced by FastQC (version 0.11.5)