FastQCFastQC Report
Wed 25 May 2016
SRR1779999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189433
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT2208311.657419773745863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2012710.624864727898519No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT68783.6308351765531874No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG38062.0091536321549044No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC23031.2157332671709786No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT10700.5648435066751833RNA PCR Primer, Index 36 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT7180.37902582971288No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC6270.33098773708910273No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC5880.3103999831074839No Hit
ATTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3730.1969033906447134No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC2850.1504489714041376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCA150.00222079870.0097770
CGTCTTA207.906087E-570.0097748
TCAACGT150.00222079870.0097768
TGCCGTA252.833929E-669.99129545
AGCCGTC301.020544E-769.9912945
ATATCCG207.916437E-569.9912910
AAGCCGT353.6943675E-969.9912944
TTTATAA150.002223125769.9912946
AACTCCG207.916437E-569.9912910
ACTCCGA207.916437E-569.9912911
GCTGTCC150.002223125769.9912939
GCCGTAT301.020544E-769.9912946
TCAACTC150.002223125769.9912933
TGTCCCC150.002223125769.9912941
AGGGGGG36150.062.26318770
CGAATGC401.0617441E-861.2423841
CGTCTAC352.9597504E-760.00837748
CTTTATA1300.059.2233962
TCCATAC308.346296E-658.34147664
CGTATTC308.346296E-658.34147648
Produced by FastQC (version 0.11.5)